ZRANB3

DNA annealing helicase and endonuclease ZRANB3

1079 amino acids

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558).

Belongs to the SNF2/RAD54 helicase family.

123.248 kDa

MPRVHNIKKSLTPHISCVTNESDNLLDFLPDRLRAKLLPFQKDGIIFALKRNGRCMVADEMGLGKTIQAIGITYFYKEEWPLLIVVPSSLRYPWTEEIEKWIPELSPEEINVIQNKTDVRRMSTSKVTVLGYGLLTADAKTLIDALNNQNFKVVIVDESHYMKSRNATRSRILLPIVQKARRAILLTGTPALGRPEELFMQIEALFPQKFGRWTDYAKRYCNAHIRYFGKRPQWDCRGASNLNELHQLLSDIMIRRLKTEVLTQLPPKVRQRIPFDLPSAAAKELNTSFEEWEKIMRTPNSGAMETVMGLITRMFKQTAIAKAGAVKDYIKMMLQNDSLKFLVFAHHLSMLQACTEAVIENKTRYIRIDGSVSSSERIHLVNQFQKDPDTRVAILSIQAAGQGLTFTAASHVVFAELYWDPGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDTLMWGMLNRKAQVTGSTLNGRKEKIQAEEGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEKQHDIRSFFVPQPKKRQLMTSCDESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKRLKLAASEDHCSPSEETPSQSKQIRTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNSELPYCEMCETPQGSAVMQIDSLNHIQDKNEKDDSQKDTSKKVQTISDCEKQALAQSEPGQLADSKEETPKIEKEDGLTSQPGNEQWKSSDTLPVYDTLMFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMKQRIIRKSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLLEDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK

ZRANB3

DNA annealing helicase and endonuclease ZRANB3

1074 amino acids

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (By similarity).

Belongs to the SNF2/RAD54 helicase family.

121.768 kDa

MSRGHNIKKSLTPQISCSTSESYKQLDFLPDKLRAKLLPFQKDGITFALRRDGRCMVADEMGLGKTVQAIGIAYFYKEEWPLLIVVPSSLRYPWTEEIEKWIPELSPEEINVIQNKTDVGRISTSKVTVLGYGLLTTDAETLIDALNNQNFKVVIVDESHYMKSRSATRSRILLPIVQKAKRAILLTGTPALGRPEELFMQIEALFPQKFGTWTEYAKRYCNAHVRYFGRRSQWDCRGASNLNELHQLLSDIMIRRLKTEVLTQLPPKIRQRIPFDLPSAAAKELNSSFEEWEKLMRDPYSGATETVMGLITRMFKQTAIAKAGAVKDYIKMMLQNDSLKFLVFAHHLSMLQACTEAVIENKTRYIRIDGSVPSSERIHLVNQFQKDPETRVAILSIQAAGQGLTFTAATHVVFAELYWDPGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDTLMWGMLNRKAQVTGSTLNGRKEKLQAEEGDKEKWDFLQFAEAWTPNERSEELRDEMLFTHFEKEKQRDIRSFFLPNAKKRQLETSCDESRVSQEKNTIVPADPVKTATRGDESDLEPEAKKLKSVAIDDPCRPPEEQPCRPGQAEALLTFGICKAKAQATTPAFCGEGWQCAFCTYINNSVLPYCEMCENPRGGAVPQIDSLNQTQNKNKNEKDDSQDTSKKIQTSSDGEKQVLAHSTPEPLAKSKEEISTTESEDRLTPQPGDEQLKNWPVYDTLMFCASKNTDRIHVYTKDGNQMNCNFIPLDIKLDLWEDLPASFQLKQNRSLILRFVREWSSLTAMKQKIIKKSGQLFRSPVLALEEIAKQQTKQNSTKRYITKEDVAAASMDKVKNDGGHVRLITKGPKPGDPSTKEFLEGGECVPFLNPCTAQGDLILKASTSKGYLQAVDNEGNPLCLRCQQPTCQTKQERKADAWDSRFCSLKCQEEFWIRSNNSYLRAKVFEIEHGVCQLCNLNAQELFLRLRDAPKSQRKSLLDATWTSKLPLEQLNEMIRSPGEGHFWQVDHIKPVSGGGGQCSLDNLQTLCTVCHRERTAQQAKERSQVRRQSLASNHGSDITRFLVKK

Zranb3

DNA annealing helicase and endonuclease ZRANB3

1069 amino acids

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (By similarity).

Belongs to the SNF2/RAD54 helicase family.

120.896 kDa

MPTAGSKKKAPTPQISCLTSESYTQLDFLPDKLRTKLLPFQKDGIVFALRRDGRCMVADEMGLGKTIQAIAIAYFYKEEWPLLIVVPSSLRYPWIEELEKWIPELEPEEINVVMNKTDIGRIPGSRVTVLGYGLLTTDAETLLDALNTQNFRVVIVDESHYMKSRTAARSKILLPMVQKARRAILLTGTPALGRPEELFMQIEALFPQKFGTWIEYAKRYCNAHVRYFGKRRQWDCRGASNLSELHQLLNDIMIRRLKSEVLSQLPPKVRQRIPFDLPPAAVKELNASFEEWQKLMRAPNSGAMETVMGLITRMFKQTAIAKAGAVKDYIKMLLQNDSLKFLVFAHHLSMLQACTEAVIESKSRYIRIDGSVPSSERIHLVNQFQKDPDTRVAILSIQAAGQGLTFTAASHVVFAELYWDPGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDSLMWAMLNRKAQVTGSTLNGRKEKLQATEDDKEKWGFLQFAEAWTPSDSFEELKDSVFTHFEKEKQHDIRSFFLPKLKKRQLETTCDDPEAFKEKITVASDPRKMATSDSTADKNGCEPEAKRLKSLSTEDHSSALEEGPSLQARATSMEVVHEVKPPLASPALPEKGWQCGFCTFLNNPGLPYCEMCENPRSRAAGRNHLQDNNKNDEDAAQESTSKSDQAGLECERQCPERLEAEQSANSKEEALEGGGEDRLPSQPEIGQLNNSGTLPVRETFMFCASRNTDRIHLYTKDGKPMNCNFIPLDIKLDLWEDLPATFQLKQNRSLILRFVREWSSLTAMKQRVLRKSGQLFCSPLLASEEITKQQAKENNTRRYITKEDVAKASMNKVKSDGGHIRLITKESMTQDSSLKKIDSACVPSLNPCPADLTVEPSPSKGYIQAVDKEGRPLCLRCQHPTCQPEQTAKASAWDSRFCSLKCQEEFWIRSNNSYLRAQVFATEHGVCQHCGVDAQELFLRMRDAPKSHRKSLLNAAWTAKLPLEQLNEMLRNPGEGHFWQVDHIRPVYEGGGQCSLDNLQTLCTVCHKERTAQQAKERSQVRRLSLATKHGSDITRFLVKK