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TTHA1902

Gene
TTHA1902
Protein
1,4-alpha-glucan branching enzyme TTHA1902
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Length
520 amino acids
Function
Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha-1,4-linked oligosaccharides of 2-7 glucose residues, beta-cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl-beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin).
Similarity
Belongs to the glycosyl hydrolase 57 family.
Mass
59.169 kDa
Sequence
MARFALVLHAHLPYVRAHGMWPFGEETLYEAMAETYLPLIRVLERLRAEGVEAPFTLGITPILAEQLADARIKEGFWAYAKDRLERAQGDYQRYRGTALEASARHQVAFWELTLDHFQRLSGDLVAAFRKAEEGGQVELITSNATHGYSPLLGYDEALWAQIKTGVSTYRRHFAKDPTGFWLPEMAYRPKGPWKPPVEGPPEGVRPGVDELLMRAGIRYTFVDAHLVQGGEPLSPYGEAALGPVESQEATYHVHELESGLRVLARNPETTLQVWSADYGYPGEGLYREFHRKDPLSGLHHWRVTHRKADLAEKAPYDPEAAFAKTEEHARHFVGLLERLAGRHPEGVILSPYDAELFGHWWYEGVAWLEAVLRLLAQNPKVRPVTAREAVQGPAVRTALPEGSWGRGGDHRVWLNEKTLDYWEKVYRAEGAMREAARRGVLPEGVLRQAMRELLLLEASDWPFLMETGQAEAYARERYEEHARAFFHLLKGASPEELRALEERDNPFPEADPRLYLFREA