TSEN2

tRNA-splicing endonuclease subunit Sen2

465 amino acids

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.

Belongs to the tRNA-intron endonuclease family.

53.247 kDa

MAEAVFHAPKRKRRVYETYESPLPIPFGQDHGPLKEFKIFRAEMINNNVIVRNAEDIEQLYGKGYFGKGILSRSRPSFTISDPKLVAKWKDMKTNMPIITSKRYQHSVEWAAELMRRQGQDESTVRRILKDYTKPLEHPPVKRNEEAQVHDKLNSGMVSNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL

Tsen2

tRNA-splicing endonuclease subunit Sen2

463 amino acids

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).

Belongs to the tRNA-intron endonuclease family.

52.952 kDa

MAEAVFRAPKRKRRVYESYESPLPIPFSQDQSPRKEFRIFQAEMISNNVVVRGTEDMEQLYGKGYFGKGILSRSRPNFTISNPKLAARWKGVQTDMPIITSEKYQHRVEWARDFMRRQGHDESTVQKILTDYTEPLEPPYRERKGESPQHEPLSSKADSSLEGREGKDELSVTTGGAGQSDDLQGLNTHSDCRQEGPGHATLTVASPSSLNGHAIEDPEALSQIPCCSQEALGQQDDLWPEASSQIAGESRAAHEYVLIEEELCDVQEGAAPHDELLKRKRLVCRRNPYRIFEYLQLSLEEAFFLAYALGCLSIYYEKEPLTIVKLWQAFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDNFEGSLRRPFSWKSLAALSRVSGNVSKELMLCYLIKPSTMTNEDMETPECMRRIQVQEVILSRWVSSRERSDQDEL

TSEN2

tRNA-splicing endonuclease subunit Sen2

461 amino acids

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).

Belongs to the tRNA-intron endonuclease family.

52.134 kDa

MAEAAFHPPRRKRRVFECYQAPFPVGPGAGDFRMCRADMVNNCVVVRSPEDMDQLYNKGYFGKGILSRSRPVYSISDPSLVSKWQGANLNVPIITSKKYQQRVQWAKSALQEQGFDGCSVTKILENYTRPLEFPFLEKGGAEQTGDSCDTVCPNTENTELSGQSSTDTGNIATSSPECQKEVCQKAYAEGDPASDSMVGSKEQEPADVKETAQTSCQMHGSCMHCGCKAKGAPDRESCNMAKSRERAPEYVLVQEEEESSLCPEEGSAREQENFVKKEKLVCRRNPFRIFEYLQLSLEEAFFLVYALGCLTVYYGEEPLTILKLWEIFSEVKPSFKTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIAELVDDNFEGSLRRPLSWMSLSGLNRTTVNASKELLLCYLIKPSDMTEEEMATPECLKRIKVQELIVTRWVSSRERSEQDDL

Tsen2

tRNA-splicing endonuclease subunit Sen2

460 amino acids

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).

Belongs to the tRNA-intron endonuclease family.

52.214 kDa

MAEAVFRAPKRKRRVYESYESPLPIPFGQDQGPRKEFRIFQAEMISNNVVVRGTEDMEQLYGKGYFGKGILSRSRPNFTIANPTLAARWKGVQTDMPIITSEKYQHRVEWARDFLRRQGHDESTVQKILTDYTEPLELPCREEKEETPQHEPLSSKADSSLEGRVEKDELPVTPGGAGQSDDLPGLGTHSDCLQEGPGHATLAAASPSSHNGHVAEDPEVLPQETLVPQGGLWPEASSQAAGEKRAAHEYVLIEEELCGAQEEEAAAASDEKLLKRKKLVCRRNPYRIFEYLQLSLEEAFFLAYALGCLSIYYEKEPLTIVKLWQAFTAVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELLDDNYEGSLRRPFSWKSLAALSRVSGNVSKELMLCYLIKPSTMTAEDMETPECMKRIQVQEVILSRWVSSRERSDQDEL