About Products Protein Database Contact

TRM5

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Ustilago maydis (strain 521 / FGSC 9021)
Length
628 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
70.133 kDa
Sequence
MSISSCSAQDGCASTAMTRTAMRQNKFATSAPASVPRLTGTEPQLGSMQGAQELLEQLKASFTSSHLIQAIGLSGRQLPLLTKDQEILPYLFNAAGVRYVQPHPNGDASQRILLLNVPASSPTPDVVLSAILKHRLELIHEFELSLGYEHLSSDQILEALLPTSIVDTDGVPTGFTIVGHIAHLNLLSVYKPFRFLVGHIILSKHIGTLRTVVNKLDSIDTQFRFFEMELLAGEADFVAQVSESDCSFQFDFRSVYWNSRLHAEHMRLIKKCRPNQVLADVMAGVGPFAVPAAKRGTWVLANDLNPSSYESLTKNAEINKVLLREGEAKPDKDGGLVATCMDGREFVRWSMLEVWKRGFAGRPMGFDGEQFDVQDDKLRQSARKMRKEQAKKNRALYAVRQAENTLEGAAESLANLSVDEPDVAATMGQVERHPERKLVDHFVMNLPAIALEFLDAFRGAYTHLATIVGKQRLLCQLELHKLDASIHRLPMIHVHCFSKDPFTPALDILTRANEALNIPRDAPYRLMAKPILPPAQTFAGLRKLCDASQSASYLSSHPNYSKYKHLESQHDFMQYVHQEWLERDAAQHTPNLSIHYVRDVAPNKQMYCLSFQCPSQVLWANTSTSQHT

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)
Length
543 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
61.029 kDa
Sequence
MLKSLCFVIRPAIVSRPQFRLPTIARLSLRQFQNQPQSVGFFTMAPLETRALALSPSATICPPSRIGTTELDRSAFDLDVQILSAVVEPGMIGKLRSHPSLKDLVLDLPKTKPIVECPAGLAPVAARDVKGLKLLRFHLSKESELPEEAKEVLKGAKALVKEVVRLGYDNWNASEILGACLPTTKSEDIPSSFTTTGHIGHMNLREEWLPFRYLIGQVVLDKNPGLRTIVNKLDTIHAQFRYFDMEVIAGDNDYIATVNESGCSFTFNFSNVYWNSRLHHEHERLISLFPPGCVIADVMAGVGPFAIPAAKKGCYVLGNDLNPESVKWMRENRLRNKVEPTLRVSEIDGFEFIRIAPLEVWTRPFDPAPPPKVSNRQRDREAKEARRKREQAKAAGQPVTETAPMSIPSQEVAIKPHPPQPPKLISHFIMNLPDSAITFLPSYVSCYTPLLAERSFIDEYGGEEEAKRKVEMPMVHCYCFTKEIEIGKAEIDILQRASTNLSFNLTPQVENYNLHHVRSVAPNKDMYCLSFRLPREVAFRHNG

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)
Length
510 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
58.198 kDa
Sequence
MEEELICRGFDSVEVLFVDVFEKSRLSVGGDGERMLQTPQTGSLMLSSLLTTRRTISSMAALQQPSRHRFPPPNNIQRLPLRPDGQQEPIKLTEAIKQQFKQSIPLLAARVPSPSITKFKTNPEIRKALLNSKNLRSVEPDPDSTDNAFKLLLLNTPHKDQIPESTLKVFQEHDIQIKNTKLDLDWEYWMADEIIERLLPDQLTDIPASFTMIGHIAHFNLRDEYLPYKYLIGQVILEKNLAIKTVVNKIDNINSQFRFFEMELLAGEPDYTVTLWQSGCRYRFDFSKVYYNPRLSTEHDLLSSMIEKDEVVVDAFAGVGPFAMRAAANRKAWVLASDLNPASVEALETNVRLNKLQGRVAVSGGDGREKIREAVRTLWLDKPFKTPNSSPLLPDHFIINLPDSSIQFLDAFRDLYHPLSDSEGFLNAVKKKSRLPLLHCYCFTKQVDEPESDICQRVSEVMKVEISPSTVARFELKFVRAVAPHKDMYRITFELPLKLLFSSSSEESRA

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Yarrowia lipolytica (strain CLIB 122 / E 150)
Length
508 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
58.62 kDa
Sequence
MKFNFWKGLWKPKSLTPTLSHRLYRRMYTPQPPLNREMTVLDRSKFTVSLNLALAKFPDPKYIAQFAKECKADILQLRSINHVQKTDDNGRAVLLRHDLDLEGGEDVASKVMSQLSPRGKELLTEAQADITKIVKDLDYDFWRADEIFYSVLPVTEKDEIPSGFSMVGHVAHLNLRSEWKDYDRLIGQVILDKNPRVKTVVNKVDSIDTKFRTFKMDVLAGEDNTEVEQHESGCRFQFDFAKVYWNSRLHTEHDRLVSLFRGEASSRERKQQERAKRENHEKSTETAVEPDNTPATAVCDVFAGVGPFAVPSGRTSLFVMANDLNPYSYEALEHNVKLNKVSEYVKCFNLDGAEYVQQSMKLLDEHRRTQPTINPVQVRKRKAGQPVVKQDIPNHYSHYVMNLPDSAIEFLWSFKGLYTTVDGLSQDTPLPHVHVHCFHKWEAGEEEPSKEETKAALLERVYKQIDVRLDPNEVGMHWVRKVSPKKDMFCISFELPKEVAWAPQVNKE

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Length
499 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
56.514 kDa
Sequence
MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFITKIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
Length
483 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
55.5 kDa
Sequence
MEEAATLQSLSISSSSPFPNNSSPVVTAANTISTSESDMFRAPVNRMMRTLDRSFFKKTVPLSAATIFDKQSIGSIKSELLQSQDLLLANRIIPVRAAREGPLENVTDHWYGRKDGKQDGRKCLLLREGIKADDVTTWSPTIQKLVEAKSVEVRPFNLLLDYDYFTYTHFNLREQYLPYKYLLGEILRDKHPQARTVINKTDDVGSHSEFRTFSYEVLAGEDDMLVTVHEQDCEYSFDYSKVYWNTRLATEHERMVSRFKKGEAVCDVMAGVGPFSIPAGKKQVFVWANDLNPYGYECLERGAAKNKVREFVKAHNMNGRDFIRFATERLYQGNPRTVVHRTKVPKAERENSPIRQRKPKAFDTEYLTCPRTFDHFVMNLPATAIEFLDAFRGLYAGMQELFEPYTDRKLPLIHVYCFSTNSEDEALERKDICERISERLGFKITPEDEGRELEIRSVRLVSPTKKMFCASFRLPAEVAFKKA

Gene
trm5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
Length
479 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
55.196 kDa
Sequence
MDSMTDSSNSLRSSDLPEMFRPPVNRAMRVLDRSFFRKTVPLSAAAVFQNSDISKVRAELHKSRDLLAVPRLNCIRDVADQEGQIKKALLLRETVKHDDKETWSPKISELVEKGRIAMRPYDLTLDYDFWTYADIISSILPEDELQEIPQGFTQVGHVLHLNLREQYLPYKYLIAEILKDKNKVIRTVINKTEDVGSHSEFRTFPFELLAGDNDLNVVQHEQDCEFRFDYSRVYWNSRLETEHRRLVEKFNKGEMVCDVMAGVGPFAVPAGKKKIFVWANDLNPHGYEVMQDAIKRNKVEGFVTPFNMDGREFIRWSAKELLETEPVTVTIHPKVRRDRKSGNKVEQAPPPHPEEYHRPVFFDHYVMNLPATAIEFLDAFPGIYAGKESLFAPHTSQRLPMVHVYCFSGHSENELDDHIDICQRISERIGYTITPEDRIGGSGNQSVELSIHNVRLVSPKKQMFCASFRLPAEVAFKKV

Gene
trm5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
Length
478 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
55.054 kDa
Sequence
MAQSTEDNTRRPRPSDLPEMFRPPVNRAMRVLDRSFFKKTVPLSAATIFKNSDISRVRRALEQSKDILALPRLNTIQDIKQDDVVKKCLLLKETIRHDDTATWSPTINELVDNGVVGVGPFDLTLDYDYWLHSDIISAVLPEELLEEVPQGFTQVGHVAQLNLREQFIPWRHLIAQVLLDKNPTLRTVIRKTEDVGSQSEFRTFPYELLAGDSDMNVIQHEQDCEFRFDFSRVYWNSRLHTEHQRLVDLFKPGEMVCDVMAGVGPFAIPAGKKKIFVWANDLNPHGYEVMQDAVKRNKVFKFVTPFNQDGRSFIRWSARALQKYDPVTVTIQPRTKRTRDASGQVKETQPPLEVYTRPKVFHHYVMNLPGNALEFLDAFIGVYAGCEELFEPHTKEQLPMVHVYCFSGHSENEVDDHIDICKRMSERLEYPITVEDRVGGAGNTELELSIHNVRLVSPNKQMFCASFRLPRAVAFRKK

Gene
trm5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Length
475 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
53.22 kDa
Sequence
MSQSEKENQGSGEGKTQDMAIFSPPVVRSGAGALNRALFTKTVNLAAAAVNDNRLISKYRKELEHSKELLRQDRLSPIVNHPDKALADQGKKCLLLSPNVKAPEPETWGAALKEGVQKKELSVIPYELQLNYDYWTYHDIITSILPEELHDDIPSGFNTAGHVAHMNLRERYIPYKKVIAEVILDKTTNIRTVINKVDNVGAESEFRTFQYEVLAGPDDMQVQVTENACSFEFDYSKVYWNSKLEAEHRRLINMFEPGEVVCDVMAGIGPFAVPAGKKGVFVWANDMNPESNKYMQVAINRNKVSQYVRPICEDGRTFIHHAADSVLEAHKNGEHVLIAPKPPSRAKKAPKPEPKRVDIPPTISHFVMNLPATAIEFLGCYRGVYAGHEDLFSAESGRKLPLVHVHCFSFKADDETPLNDICERITKYLGFPVKPGNPDVEGEVAVHDVRDVAPAKRMFCASFRIPREVAFAERV

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211)
Length
475 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
53.947 kDa
Sequence
MKYIFAPPANKGLQQLNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSMEASEINDLPNPLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETVGHIAHLNLRDRYQPFKHIIGQVILDKSSHIKTVVNKLDNIDHTFRFFQMEILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSLEDLNNPAIWNKITKQKPTSNDKKRNRKVESPTVAPLTDQPAISGIRHFKHYVMNLPATAIEFLDAFHGLYSGMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLCISFRLPSALAFAEPKILGKRKGLETEENLVSQSDVSKSSDNILEKDT

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Nectria haematococca (strain 77-13-4 / ATCC MYA-4622 / FGSC 9596 / MPVI)
Length
465 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
52.51 kDa
Sequence
MDKNSQLRDMNLFRAPAARAAKTLDRSVFAKTLNAAAASISENRLLSKYRKELEKTQEVLFMERFNPILPDPDPSLASQGRKCIVLSPQIKPASPETWSPTLQEASKLGELKVVPYDLEITYDFWNYFDVVKSILPEELHGEIPSGFNTVGHVAHLNIRDQYLPYKNIIAQVLLDKNPHIKTVINKIDNVGSENEFRTFAYEVLGGPDNMNVEVSEAGCIFRFDYSKVYWNSKLDTEHKRITSFFKPGEVVADVMAGIGPFAVPAGKKGVFVWANDKNPESHRYLEDAIQKNKVWEFVKPFNHDGHDFIRTSADLVLEASKRGDCAVIKPPRPPRKSAAPPPEPVRVPVPPTISHFVMNLPASAIEFLHNYRGLYHGHEDLFEPHTETKLPIVHVHCFSAKMDDDTPLKDICERIHKEIGVMLKPGDAEKEGEVLIYDVRDVAPAKRMFCASFRLPREVAFAARA

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003)
Length
457 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
52.203 kDa
Sequence
MTTRHLYLDTSPPAYTGSRTELDKSAFHKTLPVLAVRVRPEKVGKFLKGDAMKRALLDIPKVRTVVSDPKEEGNRLVLLRMPNEGDIPPEALELIKTESNGLTEFNVNLDYNYWTADEILQSFLPEELREGAPSGFAMVGHIAHLNLNEEYLPYKYIIGQLILEKNNRVRTVVNKINSIDTQFRFFKMELLAGEPDYVVEHHESDCRFMFDFTKVYWNSRLHTEHDRLIQVFQPEEVVADVFAGVGPFAIPAGKKGCGVLANDLNPESYKYLAINAKNNRVDDTVKAFCEDGREFIQKSVSRLWEEPLPAYTGPKQSRVQEEKERRRLQRLKAEGQTVPIPPSEKQHGRRRISHFVMNLPDSAISFLDAFRGLLSGAEPALREQYSTMPMVHCHCFTREVDSRVNAEGDIRKRVEEKLGGALTSETSFHFVRSVAPNKDMYCISFRLPPEVAFGERM

Gene
trm5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Length
450 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
51.748 kDa
Sequence
MHLQIRTVHNMAELLHPNKVEMKPFLKNLDKNLFKKTYNLVAAKVSPKKVGLLNKVCKKDLLDWPRVKHVYQQNNEKLVLLNRDASLDGTRGLSDATVSFIKENNIELVPFQLTLDYDYWRADDILDAILPPGEKEDHPSGFTAVGHIAHMNLREEWLPYKYIIGKVILDKNPSIETVVNKTDTIDTKFRTFQMEVLAGKDDFIVTQSESNCKFRFDFSKVYWNSRLSTEHDRLIQQFQPGDAVCDVMAGVGPFACPAGKKNVIVFANDLNPYSYESLVENIFLNKVANFVKAFNQDGREFIRSSVQKLLGFSKDEKAITVFPPRKRARKLEENKDPVRQDIPIPPVFSHYVMNLPGSAIEFLDAFKGCYYGLEYLFKDRSLPKVHVHCFCRFPDPEEDLINRIYASLGYRFSPEEVDFYYVRKVAPNKDMYCCTFTLPGSIIFAKPVSG

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876)
Length
449 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
51.86 kDa
Sequence
MSKFSPPINRNMVELDRSFFHKEVPLLAAYFPNPKFLGQFVKSCQNDILYVQTVKHIISMDDSKAILLRDDVKSISDLNPETQLKINEFGIILKPYTLKLDYSFWKSEEILKSILPENLIDDVPSGFSQAGHLAHINLRDEYKPFGKLIGQVILDKNPSVLTVVDKVNTIANKFRTFPLELLAGEPNYIVEQSESGCKFKFDFSKVYWNSRLSTEHERIIGKFNSGDVVGDVFGGVGPFAIPASKKNVIVLANDLNPESYKYLQENIKINKVEPFIKPFNLDGREFIRKAPELLLQWHNSQNGIIEKKIIKKVSIDDNKTKKNFERKPIIETTKIPKFYHHFVMNLPDSALTFLDEFIGLYGSNPQLKTDPEFKLPIIHVHCFEKFENNENPTPEELHNRVYEKICKLIQFPLNKEKMEFHEVRMVSPTKPMFCVSFELPEEVAFKQSK

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Candida albicans (strain WO-1)
Length
449 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
51.861 kDa
Sequence
MSKFSPPINRNMVELDRSFFYKEVPLLAAYFPNPKFLGQFVKSCQNDILYVQTVKHIISMDDSKAILLRDDVKSISDLNPETQLKINEFGIILKPYTLKLDYSFWKSEEILKSILPENLIDDVPSGFSQAGHLAHINLRDEYKPFGKLIGQVILDKNPSVLTVVDKVNTIANKFRTFPLELLAGEPNYIVEQSESGCKFKFDFSKVYWNSRLSTEHERIIGKFNPGDVVGDVFGGVGPFAIPASKKNVIVLANDLNPESYKYLQENIKINKVEPFIKPFNLDGREFIRKAPELLLQWHNSQNGIIEKIIIKKVSIDDNKTKKIFERKPIIETTKIPKFYHHFVMNLPDSALTFLDEFIGLYGSNPQLKTDPEFKLPIIHVHCFEKFENNENPTPEELHNRVYEKICKLIQFPLNKKKVEFHEVRMVSPTKPMFCVSFELPEEVAFKQTK

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)
Length
441 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
49.978 kDa
Sequence
MFAPPAARAMKVLDRSFFKKTIPTSAARIFNAKDISRCRKELTASRDTLPNNRVDPIRPDVDSERAQKGGKCLVLRPEVVHNDRTTWSPKLRDLEQDGTLGVIPFQLDLDYDFFTYSEITSAIIPPPETKQDDEIPQGFALAGHVAHLNLRERYWPYKYLIADVLADKNPMVKTVINKLDNVGTENAFRTFQYEVLHGPDDMNVELREQGCTFKFDFAKARQYATIMAGVGPFAIPAGKKKCFVWANDLNPESYKSLEDNIRINKVGDFVTPRNTDGADFIRQSAIDLLKSERSIPIYPKVKFSRSAPPTQKPVPDRTLVQPRTFQHYVMNLPASAITFLPSFIGLYSNIPGLSIGEAKKLFAPHTQQKLPMIHVHCFSTKSDDNVAETKEICAEISRQLQCEMTPETPDVNIHDVRDVAPKKRMFCASFRLPEEVAFREI

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Tuber melanosporum (strain Mel28)
Length
428 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
47.946 kDa
Sequence
MSRLFLPPINRAMRTLDPSFFQKTIPLTAAHVLDGRNIPRVTKECARDLLRVPRLRSVITQKDDAGMERKLLLLRPEVKHDDLSTAQPKTREFVEERIIKLEPYTLHMKYESWNYEEIMNAILPEDLEEIPCAFTQAGHLAHLNLREQYLPYKHLIATVLLDKNPNVSTVVNKIEDVGTGSVYRTFPMELLAGQDNTNVEVRESGCVFRFDFAKPGEAVADVMAGVGPFAIPAAKQRVFVYANDLNPESYKSLVGNIHVNKVSQFLTPHNLDGANFIRESIRTLLSRSRNPSTSKVSIPTPGKRGQKRPPPPTVIPVPPTFSHFVMNLPASATTFLGAFRGAYRGLEDVVTGQEMLPIIHVYTFHKSSVTENAAGDICADISRHLGREMGEGDLENLENVRLVSPNKTYYCVSFRLPANVAFEDAQVA

Gene
TRM5
Protein
tRNA (guanine(37)-N1)-methyltransferase
Organism
Encephalitozoon cuniculi (strain GB-M1)
Length
360 amino acids
Function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Mass
41.015 kDa
Sequence
MDVMDDVKSVLCLKALEASNERASDCIQRAGASIRKIPRIPSIIRVKKKYSTFRVLTDTSHDLLVLVNEGFSDGEYVPINVELELCYEYFTYSEVLRRILPEEVQTPSSFEIVGSIVHLNLDGEQMKHKNIIGRVVHDKTGKTVITKIGQISNAYRSFDLEVIGGDPVLETIHREGDILFCIDYRSVYWCSKLQSERAILAGKFKAGEVLCDPFCGVGPVSLPALKKGCRVYSNDLNLRAIECLEKSIKINRLDPRNIEIFNLSANEFLEKMAGRKIDHFFLNLPEHSLDYLRRISAWEGNPLVHCYFFCRSNEDVVQYIFSRTGLRVDPGMLKVVRKVSPSKYMYKLETSSLFLRRGLG