Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Topoisomerases 1 enzymes (TOP1A and TOP1B) are essential for plant survival (PubMed:12215507).
Sequence
MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKSSPPMRSPLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRDDRSVVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNIDRNGLKPKTEGHSSQAPAKRPLEKGSSSNQSSVKRPKLSEPARPVKVEQGSHISATQDAKGKNLDASKPLRANQATVKEDNSDGDDHVPIASRMKSDSSNNKSSSAKPSSSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLPSGDGQKKWKTLQHNGVIFPPPYKRHGVKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVDPEFRFCSSTDE