Protein
Hybrid PKS-NRPS synthetase TAS1
Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)
Function
Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein (PubMed:26503170). TAS1 alone is sufficient for TeA synthesis (PubMed:26503170). The NRPS part performs the condensation of isoleucine with acetoacetyl-CoA and the final cyclization step is conducted by the KS domain (PubMed:26503170).
Similarity
Belongs to the NRP synthase family.
Sequence
MYNRCCRKVWIQGRVAYRPAYIRQSPPQQTLYRGIDLLPFTTIVMDHQSGFQNPSPSGGLMFSASAKRFISRIVGFVGRGAEVQQKAVGLCPLKTERRRAAAGGLLLPTGLRMGRSIIMSVRPLPFLTGPAPSPDTAAGFKPSPPTGNLVSVSPLSKAQMALWFDYLQHPTSTHYFLTLKVELDKQPLSLDKIIQVIRGLGKQHAMLRTTFHVDTDTDDMSKSYMAVHDDSWDQEIHVLMNDAQLYEALRKPFQLSSESPVRWVVQMKLQPGSARSTYTVYAAGHHIGVDGASMSVLSNQLLEAVASEVEDQPDHSGPHYGDYIQRQARYLRSSAGAAAGRFWLSQLRHTQPFRWRMEPPEEINTPNYRQLDTWNFFPTAEIQEWGNLYKTSWFRVATSIVGLVTAAMAEPQAHHDHALMVAFGARPRGFENNVSHMANTMPVKFPLSSLLRDDATFSDAVKAMGRNVSTAKKHENFPFMSLMEQANRHMDPTLLDFKVAITYSPKLANKSCELFPVEGIWDLFFCFLEQEDGVALGVISNPRVFGAEALGQLQSLFNEVFALSKARPSFKLSDLAFLQNRTPARFISGPALDDVESISKSRVYRLIKARAASQPDLVALMSAEKGVQMTYRELAAQSSQVAHFLQKQRLCKGDAVLVHLERGFAQIVWILGVMEAGACYVALDKTWPAARKEAILRTANGKLLVTDDEQMDFEKQDTTVVFLAPSAAEIASMPQSTCECEVADDDLAYVVFTSGSTGQPKGVMVEHSNLSHYVSATRSLVKTGPHSRMLQLASFAFDAIVLEYAVTLAHGGTLCFANHPEVLVGEYLADVIDSNQVNFFHCTPSVLSTLPAGRRLPSLRIVSVGGEASPPGLLDHWRKRVELLHAYGPTECTVICTLESLTQDESTQTAIDATVIGKALPNLDIRICEEGKLEPLAPNQVGEICVVGPQVSRGYMGQEELTASKFHNITLADGHPSRLYRTGDKGFIDDDGKLHIQGRIGNREIKVRGYRLDLYEVEKNVMAFDPEVTQVSIQQVGESLVALVVPASIDCDRIRSKLLKDMPRYAVPTRFIRVASLPLNTNGKIDHTQASSLAAELVMHDTVLPTVDATPTPTAAVRAVGVTEENLRLKTKENGMERQEMLRRHLTAEVTALWAKLLGSSRQFDPEVGFFDAGGHSLLLTQLHKLIKERFGTGSRPSLLDIFSMSSIRKQVDCLMGIVDQDAMLGSEPTGGSSSRSQSRRSAETSSSSTSAPSSVPVDAERNLYAIVGISCRFPGANTAEQLWNVLMEQRDAITTFCPAENLGFALEENSVFVPRYGMIDALKDFEPSAYSMSDAEAQTIDPQKRVFLDVAADALADAGTSASPGNPLDPVGVFVGAATNTFLSSRDNPGSKPPGDEEPQSFANHYQQLLDCPIGTFASFKLNLTGPVVTLNTACSSALAALHLACASLSHGDCNAAVVGGVSMAYPQEGGYVTARPGGDSSAVFSPSGVCHPLDSRADGCVPADGAAALVIKRLADARADGCRVYAVIEGVAVSADGSDDKAGLGVPSSSGQSRTVEAALRRAGPQALSRLRYVEMHGSGTPWGDALEVQGLKMAFDRLSKTGAAEQSGTGRAQPEADRIYLGSNKGNCGNTEAASGLLSLIKASMALNLGVVPPLPNLAEPNPKCEFEETKFEPLGKQLALAPGDRVMNKRQRIVRSPNWISDATWFVISTVDNLLPKLTPAAVA