SSL2

General transcription and DNA repair factor IIH helicase subunit XPB

843 amino acids

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription (PubMed:8269516, PubMed:7693549, PubMed:7961739, PubMed:8202161, PubMed:7813015, PubMed:8631896). XPB/SSL2 acts as a double-stranded DNA translocase promoting DNA opening by tracking along the nontemplate promoter strand, rotating and inserting DNA into the Pol II active site cleft, leading to DNA unwinding. May also use this translocase mechanism during DNA repair rather than physically wedging open damaged DNA (PubMed:25775526).

Belongs to the helicase family. RAD25/XPB subfamily.

95.341 kDa

MTDVEGYQPKSKGKIFPDMGESFFSSDEDSPATDAEIDENYDDNRETSEGRGERDTGAMVTGLKKPRKKTKSSRHTAADSSMNQMDAKDKALLQDTNSDIPADFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLAEQAQDFLVTIAEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRLSKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQADILQMLLNDSVIGPLRIDSDHQVQPPEDVLQQQLQQTAGKPATNVNPNDVEAVFSAVIGGDNEREEEDDDIDAVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAKLGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSDNVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELLQEVLLKNEEAAGIEVGDDADNSVGRGSNGHKRFKSKAVRGEGSLSGLAGGEDMAYMEYSTNKNKELKEHHPLIRKMYYKNLKK

SSL2

General transcription and DNA repair factor IIH helicase subunit XPB

687 amino acids

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.

Belongs to the helicase family. RAD25/XPB subfamily.

78.771 kDa

MTRCGWHTCTRVRPRMGETLLPRKASQHEFEPEEIRMKQEHTECPLLVSHNGIIILETFTANAKQATDFLIAIAEPVSRPAHVHEYRITPYSLYAAVSVGLTTEDILSTLDRFAKNTVPDTIVRFVRECTLSYGKTRLVFKGGRFLVEAATREVFDVLTGDAEISRLRAAGTVRDADARYVFEVRVDCIEQVRRRCIEIDYPMIEEYDFRDDVALRSLDMDLRDTVSIRTYQEVSLNKMFGNRRARSGVIVLPCGAGKTLVGITAMCTIKKPCIVLCTSGVSVEQWRQQVLAFSTVSADAVSRFTSERKEMFEADAGILITTYTMLAFSGRRSAEAQRVMEWLRGTEWGLMILDEVHVVPAAMFRKVVSAVSHHCKLGLTATLVREDDKIEDLNFLIGPKLYEADWQDLSMQGHIARVECVEVWCDMTAEFYREYLGQDPRRRRVLSIMNPAKFQTCEFLIRKHEALGEKIIVFSDNVLALRTYALKLGKPFIYGPTGQTERMRILRQFQTNPAINTLFLSKVGDTSIDLPEASCLIQISSHFGSRRQEAQRLGRILRAKRRNDPGFRVYFYTLVSKDTEEMYYSRKRQQFLVDQGYTFRTVTALEGFRDTDVRVFRGKAEQRELLATVLLASEEDLESEESEDGDEAAADRRHLRTRGGAEDTVPASRTRTERHLLFRKMHKRHRR

SSL2

General transcription and DNA repair factor IIH helicase subunit XPB

672 amino acids

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.

Belongs to the helicase family. RAD25/XPB subfamily.

76.626 kDa

MNEVVIPKKATKHQFPYEELVLKEDGESHPIWVNYDGLIILETFRESSRQASDFLIAIAEPMSRPLQIHEFQITAYSLYAAVSVGLTTSDIIETLDRFSKNFLPRSVRVFITECTLSYGKVKLVMKESSFFLETANESVYKMLSSDAVIRSHTIGKSKEGGGLSIEVEEVELVKKRCIEIDYPLIEEYDFRNDKVLRSLQIDLKPTTIIRSYQEICLNKMFGNGRARSGIIVLPCGSGKTIVGITAISTIKKNCLVLCTSAVSVEQWKQQTLQFTNMAPDGVGRFTSDHKEWPKDDSGIVITTYTMLAYTGKRSHEAQKIMDLIRRTEWGLLVLDEVHVVPAMMFRRVLSLVSHHCKLGLTATLVREDDKIEDLNFLIGPKLYEADWQDLSAKGHIARVSCIEVWCGMTGDFYREYLSQPTRRRRLLSIMNPTKFQVCEYLINKHESRGDKIIVFSDSVYALKAYALKLGKPFIYGPTGQTERMRILKQFQTNPVINTIFLSKVGDTSIDLPEATCLIQISSHFGSRRQEAQRLGRILRAKRRNDPDFKVYFYSLVSKDTDEMFYSSKRQQFLIDQGYTFTILTDIPEVHENEHCVYKTKGQQRELLAGVLLASEKELESEESEDSEGIVYSTTKLKSLSGGDEMAYIERKAPQGRTLAKGSKKSRKWNPRP

SSL2

General transcription and DNA repair factor IIH helicase subunit XPB

659 amino acids

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.

Belongs to the helicase family. RAD25/XPB subfamily.

75.649 kDa

MNNVMLPKKAQKHQFPVEEIILKDDHENFSLWINYDALIILETFKQNSKQASDFLIAIAEPVSRPKYIHEYQITAYSLYAAVSVGLSTDDILSTLSYFSKNIMPRSVKNFIVECTLSYGKIKLVIQFNKYFIEAANNDILKKLIDDKIIRSNLFVTDNSVEVINVELVKKRCIEIDYPLIEEYDFKNDTVLESFFIDLKPSTMIRSYQETCLNKMFCNGRARSGIIVLPCGSGKTLVGITALTTIKKSCLILCTSAVSVEQWRQQTMLFTNISGDNICRFTSEYKEWYKDKCGIIITTYTMLAYNGKRSPEAQKIMDLIQSTEWGLLLLDEVHVVPAMMFRKVISLVTHHCKLGLTATLVREDDKIEDLNFLIGPKLYEADWQDLSAKGHIAKVECFEVWCGMTGEFYKEYLIQTSRKKRLLSIMNPTKFQACEYLISRHEAKGDKIIVFSDSVAALKSYALKLGKPFIYGPTGQTERMRILKQFQTNPLINTIFLSKVGDTSIDLPEATCLIQISSHFGSRRQEAQRLGRILRAKRRNDPDFKVFFYSLVSKDTDEMFYSSKRQQFLVDQGYSFNIITEIPEIYKNENRVYKSKSQQKELLVSVLLSSDKELESDTEADNEEIGVYSGILKGSTGVDGMAYMETNERNDLGKNKRRRK

SSL2

General transcription and DNA repair factor IIH helicase subunit XPB

609 amino acids

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.

Belongs to the helicase family. RAD25/XPB subfamily.

70.329 kDa

MKENSEDCPLWINYDGLIILEMFRENSQQATNFLIAIAEPISRPENIHEYQITPYSLFAAASVGLTTDQITNTLQKFSKNIIPRNVKNLISDCTLSYGKLKLVRQSQKFFIEVYNDKIFNFITSDEILKSFIVNSNIDELKIEITNVEKIKKRCIEIDYPLIDEYDYTAYESVNMIKNLHIDLKPSCHIRSYQEISLNKMLGNGRARSGIIVLPCGSGKTLVGITAISTIKKSAIILCTSAVSVEQWKQSILLFTTINPYSVSRFTSDCKEWFENYNVENTSQGGILITTYSMLSFSGKRSYDVQRIINKIFAYNWGIMILDEVHVVPAQMFRKVVSSVLHQCKLGLTATLVREDDKIEDLNFLIGPKLYEANWQDLSDKGHIAKVECSEVWCEMTAEFYREYLIQDTSKKRLLSIMNPVKIQMCEYLIQKHEAQGDKIIVFSDSVFALKEYAIKMKKPFIYGPTSQTERMKILKQFQINSKINTLFLSKVGDTSIDLPEATCLIQISSHFGSRRQEAQRLGRVLRAKKRNNPNFKAYFYSLVSKDTEEMHYSAKRQQFLIDQGYSFKTIIGFNDMYYNETRLYKTKQEQKELLFNLLSKNLSSDETDK

SSL2

Protein STRICTOSIDINE SYNTHASE-LIKE 2

376 amino acids

Belongs to the strictosidine synthase family.

41.475 kDa

MMKLLLVVATSVALIFSVTDLSGEGPKHGGESMLTVQIPDFRLIPTTGALGPESFVFDFFGDGPYTGLSDGRIVKWLANESRWIDFAVTTSAREGCEGPHEHQRTEHVCGRPLGLAFDKSTGDLYIADAYMGLLKVGPTGGVATQVLPRELNEALRFTNSLDINPRTGVVYFTDSSSVYQRRNYIGAMMSGDKTGRLMKYDNTKQVTTLLSNLAFVNGVALSQNGDYLLVVETAMCRILRYWLNETSVKSQSHDNYEIFAEGLPGFPDNIKRSPRGGFWVGLNTKHSKLTKFAMSNAWLGRAALGLPVDWMKVHSVWARYNGNGMAVRLSEDSGVILEVFEGKNENKWISISEVEEKDGTLWVGSVNTPFAGMYKI