Protein
Beta-1,4-mannooligosaccharide phosphorylase
Organism
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
Function
Catalyzes the phosphorolysis of beta-1,4-mannooligosaccharides to mannose 1-phosphate (Man1P) and shorter mannooligosaccharides. Can also catalyze the phosphorolysis of 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc), but shows higher activity toward longer mannooligosaccharides. Involved in a mannan catabolic pathway which feeds into glycolysis.
Similarity
Belongs to the glycosyl hydrolase 130 family.
Sequence
MKTQIINGVSLPNIPWQDKPADCKDVIWRYDANPIIPRDQLPTSNSIFNSAVVPYESEKGKFAGVFRVDDKCRNMELHAGFSKDGIHWDINPDRIVFEQAEKSTEEVNQWGYGYDPRVCFIEDRFWVTWCNAYGWKPTIGVAYTFDFKTFYQCENAFLPFNRNGVLFPRKINGKYVMFSRPSDSGHTPFGDMFISQSPDMKYWGEHRHVMGPLRAWESKKIGAGPIPIETSEGWLCFYHGVLESCNGFVYSFSACILDKDEPWKVKYRCAEYLLSPQKIYECVGDVQNVTFPCATLVDADTGRIAIYYGCADTCVSMAFTTVDDVVDYVKSHSSV