Protein
3-hydroxylaminophenol mutase
Organism
Cupriavidus necator (strain JMP 134 / LMG 1197)
Function
Catalyzes the isomerization of 3-hydroxylaminophenol (3HAP) to aminohydroquinone, a step in the degradative pathway of 3-nitrophenol. The enzymatic reaction is regiospecific since it leads to the formation of aminohydroquinone exclusively, without producing the isomeric 4-aminocatechol. Can also isomerize other hydroxylaminoaromatic compounds, such as hydroxylaminobenzene to a mixture of 2-aminophenol and 4-aminophenol, 4-hydroxylaminotoluene to 6-amino-m-cresol, and 2-chloro-5-hydroxylaminophenol to 2-amino-5-chlorohydroquinone. Does not act on 4-hydroxylaminobenzoate.
Similarity
Belongs to the glutamine synthetase family.
Sequence
MAQSVADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPTSHFDDDKFESGHAFDGSSIAGWKGIEASDMLLMPDSNTAFIDPFYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDGVTWNVDMQGCFVKVHSEEAPWSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTRFSTLVQRADWTQLQKYVIQNVAHTYGKTATFMPKPIVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLMNPYLGFSALLMAGLDGVMNKIHPGEAADKNLYDLPPEEDAKIPTVCNSLDQSLEYLDNDREFLTRGGVFSNSMLDAYIELKMEEVTRFRMTTHPVEFEMYYSL