RFS

Galactinol--sucrose galactosyltransferase

798 amino acids

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Able to utilize D-ononitol and D-pinitol as acceptors. May also act as a glycoside hydrolase.

Belongs to the glycosyl hydrolases 36 family.

88.717 kDa

MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSRNFLVNGHPFLTQVPPNITTTTTSTPSPFLDFKSNKDTIANNNNTLQQQGCFVGFNTTEAKSHHVVPLGKLKGIKFTSIFRFKVWWTTHWVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQPGLNDYVDMSVESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPVTERDGMNRTSAGEQMPCRLIKYEENYKFREYENGDNGGKKGLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFKEKKLSLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDYEDKMVRVQILWPGSSTLSLVEFLF

RFS

Galactinol--sucrose galactosyltransferase

783 amino acids

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers. Specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors. Able to utilize sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose as acceptors. May also act as a glycoside hydrolase.

Belongs to the glycosyl hydrolases 36 family.

85.533 kDa

MAPNLSKAKDDLIGDVVAVDGLIKPPRFTLKGKDLAVDGHPFLLDVPANIRLTPASTLVPNSDVPAAAAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMMILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVFRSAVYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVWEGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQENYKFREYKGGMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEMVCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFSGVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGGGGGDRFAVYFVEARKLQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYEDGIVTVDVPWTGSSKKLSRVEYFY

rfs

NRPS-independent siderophore synthetase rfs

634 amino acids

NRPS-independent siderophore synthetase that catalyzes the rhizoferrin biosynthesis from citrate and diaminobutane via an ATP-dependent condensation of citrate with diaminobutane followed by the addition of a second citrate to the monocitryl-diaminobutane intermediate (PubMed:28610916). Can also use as substrates the citrate and diaminobutane homologs oxaloacetic acid, diaminopropane, diaminobutane, diaminopentane, tricarballylic acid, hydroxylamine and ornithine (PubMed:28610916). Forms only a mono-substituted intermediate with oxaloacetic acid and diaminopentane whereas both mono-citryl intermediates and full rhizoferrin derivatives were detected when diaminopropane, and ornithine were used as substrates (PubMed:28610916). Tricarballylic acid only forms a rhizoferrin derivative, but no mono-substituted intermediate (PubMed:28610916).

71.816 kDa

MPVASSEYQNEHYASFATTSRLVTCLVSETLVPVFFVPVKSVDRNNQFIGLCLLLRPTTVKQESELPTNITASDILTVVPLRGLPILNNERVALFNGIRCPQIDLVDFLDMLPHIYSVESSGSLKSGDSLKQKTFDTLSAILDGNKTFDLVDGYSAVQLWNHFAQDLEINSKLREQIGQELGSSILFQKYTYDNPKPLPTLNSSTIKWEQSVVEGHATHPMHKARKSFPPMPPLNPGSYDLDHPAVRLVGIPRENAILRGEYEELSAPLVNALMDAGGNHKDIRAQYQNYVFIAIHELQLPNIQEKFKDAVIFSKEHQLNVEALASLRSVARPDILPGLSVKLCLGIKISSALRTVTPFTTYFGPGFSFNVVPKLTYDHEVLAIERELGTITYRHEDSDVAKHCSSVIREALEYDPKYQDDLFIPCGALVEKIQRPDTDETLVAHVWNLDTKEKRVEFLDRYVDFALRSFLPPCLINGVAFEAHGQNTLARFDRKTGLLKGFVIRDFGGVKAHNETLKKSAGVELDILPDSCVEAHSLEEVFKLLYHTLFHCQLQRLIRVLDLHYSGEGWEIVRKYLTQYVPKDHVMWPMFMESSKVPGKCLVRMKIDELYRDYIYRPVPNMIKYEPQSVPEAI