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PF0027

Gene
PF0027
Protein
RNA 2',3'-cyclic phosphodiesterase
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Length
184 amino acids
Function
Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. In vitro, ligates 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. Ligase activity requires GTP, but GTP hydrolysis is not required for the reaction, which is reversible. Ligase activity is weak compared to the phosphodiesterase activity.
Similarity
Belongs to the 2H phosphoesterase superfamily. ThpR family.
Mass
20.995 kDa
Sequence
MRAFIAIDVSESVRDALVRAQDYIGSKEAKIKFVERENFHITLKFLGEITEEQAEEIKKILEKIAKKYKKHEVNVRGIGVFPNPNYVRVIWAGVENDEIIKKIAKEIDDELAKLGFKKEGNFVAHITLGRVKFVKDKLGLAMKLKELANEDFGSFIVEAIELKKSTLTPKGPIYETLARFELSE