orf5

Cytochrome P450 monooxygenase orf5

489 amino acids

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of brefeldin A (BFA), a protein transport inhibitor that shows antiviral, antifungal, and antitumor properties (PubMed:24845309). The proposed biosynthesis of BFA involves formation of an acyclic polyketide chain that is differentially tailored throughout the backbone (PubMed:24845309). The highly reducing polyketide synthase Bref-PKS is proposed to synthesize the precisely reduced octaketide precursor, which could then be directly offloaded by the thiohydrolase enzyme Bref-TH followed by a cytochrome P450 monooxygenase-mediated formation of the cyclopentane ring and macrocyclization to afford 7-deoxy BFA. Alternatively, the first ring annulation can also occur on the ACP-tethered intermediate before the thiohydrolase release and lactonization (PubMed:24845309). The C7-hydroxylation by another cytochrome P450 monooxygenase is believed to be the final step in the process to obtain the final structure of BFA (PubMed:24845309). In addition to the HRPKS Bref-PKS and the thiohydrolase Bref-TH, the brefeldin A biosynthesis cluster contains 4 cytochrome p450 monooxygenases (called orf3 to orf6), as well a the probable cluster-specific transcription regulator orf8 (PubMed:24845309).

Belongs to the cytochrome P450 family.

56.075 kDa

MANDVSGLGPTAFVRLLAFHLIGLFVSITVYRLFFHNLSGFRGPFIARLSSFYLAWLSAKRLHLHDEIDDLHSLYGDYVRTGPRELSIIDPQCVQVIYGSQTKCIKGPIYTLLDPRTNLSSTRDKTEHAKRRRAWDRGFSTTALHTYEPMVQELTEELMTIIDELSENPINITEWVDKYAFEVMGQLTFGKPFNMLKERKEAYFLELIRQDMNAIGYLLNLPWLSYLFLRTPGLNQNHLNFWRWIENEFAQRIARGQRRPDVFNWLHQAYLQGPQTKSDTLKLHGDGYLVIVAGSDTTASTITHLLFYLACNKALTQKLQAQLDALEGLTDESLRDVELLDACINETLRLRPAVPAGVQRETPKEGIYIGNRYIPGDTIVKVPMYTLFRDPRSFEQPNEFIPERFTTRPELVKDKSVFIPFLTGSYACVGRRLALMEVRRAVAAILCRYDIALAPGQNEEGFLDGKIDAFTLVAAPLSLKFTRRHQQKQ

ORF5

Protein P55

483 amino acids

54.886 kDa

MDKYIYVTGILNPNEARDEVFSVVNKGYIGPGGRSFSNRGSKYTVVWENSAARISGFTSTSQSTIDAFAYFLLKGGLTTTLSNPINCENWVRSSKDLSAFFRTLIKGKIYASRSVDSNLPKKDRDDIMEASRRLSPSDAAFCRAVSVQVGKYVDVTQNLESTIVPLRVMEIKKRRGSAHVSLPKVVSAYVDFYTNLQELLSDEVTRARTDTVSAYATDSMAFLVKMLPLTAREQWLKDVLGYLLVRRRPANFSYDVRVAWVYDVIATLKLVIRLFFNKDTPGGIKDLKPCVPIESFDPFHELSSYFSRLSYEMTTGKGGKICPEIAEKLVRRLMEENYKLRLTPVMALIIILVYYSIYGTNATRIKRRPDFLNVRIKGRVEKVSLRGVEDRAFRISEKRGINAQRVLCRYYSDLTCLARRHYGIRRNNWKTLSYVDGTLAYDTADCITSKVRNTINTADHASIIHYIKTNENQVTGTTLPHQL

ORF5

Capsid protein

414 amino acids

43.712 kDa

MTSNGSQPQASTPMVSAEEPAAAASVPNSTPMVSAEGPAAAVSAPNSSVVSSAPASAPTASEPVISQVQSLAPIVSGFDPNLHGRLTNEQMRQAQNEAAMQGYEEGSRRNPRLPSSTTAHNDYASMNSNPFETGTAYGGAPRVSFGSYPTFPGSGSASEPNSQRIFPQQHGVNPPAHASDLVPHQATSGGNTGTPFTLGNRAPRNATANTGGMRRRLDSVGLKNIRYEPQAGVVASNQKIRAVGVALIGMGIPEHQLTEVGVYLARHCADVGASDKSALLGTFPGSDITLEEVGTMIKQTEGCTLRQYCAFYAKHVWNLMLQTQSPPANWVGKEFKFETRYAAFDFFFGVESTASLEPADGLIRLPTQAERVANATSKEIQMYRIRSMEGTQAVNFGEVTGGKIGPKPVLSIRK

ORF5

Capsid protein

325 amino acids

Required for genome encapsidation. Forms ribonucleoprotein complexes along with TGB1 helicase and viral RNA (By similarity).

Belongs to the potexviruses coat protein family.

35.791 kDa

MSFDYTDPTFRNYPFPHYCDFDRHQHCDHDLRTNPPPTEPPSRKSKLMSTSENKGKQPLHPPPTEGFPKPPPPPSSTPTTPTPPDQTKAPEPIEKRIIHAFHAEPKTHTNGEAPPAFNPNNMNAVPLNLLNINLKYSPVTNSIANPKQTEAIGKAWVRILQIDPANVFLYAIDLARACADAGSSPEADIIGANEDLNPVVERNALAGVVRDFCPLRAFCAYYSRVVWNLMIKADQPPANWMKSGIDEGAKFAAFDFFHGVLSPASLYVPLERHPTAAERIANQAMFAVKIANAPGNGSELTMDHVAFTKGRITADSKPRPTPFNT

ORF5

Capsid protein

293 amino acids

Required for genome encapsidation. Forms ribonucleoprotein complexes along with TGB1 helicase and viral RNA (By similarity).

Belongs to the potexvirus capsid protein family.

31.635 kDa

MSESKAETPSKSAEKGVASLSTSAPPSSTTPTAQAKQTPPPVATTARPMASRLPRTIAAEGGGTEKKQSHLAEDRIAQYLPKQDAVDHSNLAALLQPFTEASYEREFDVKVNGIASKAELTAVAETWASRLNVPKENSAVLAQEIAIHCYHNGSSEQTDFNLKSSQVAGLNLEAAVGVIKEILTLRQFAAYYATFVWNWGIKNEIPPANWVAKGYTDETKYAAFDTFSYVGSPLGLRITPTRKPTNNEYMAASVNAREKIIQSRGKGMVTNSPMFSDGTTHQGIPLHPKLPLS

ORF5

Capsid protein

262 amino acids

Required for genome encapsidation. Forms ribonucleoprotein complexes along with TGB1 helicase and viral RNA.

Belongs to the potexvirus capsid protein family.

28.487 kDa

MNEEDLNRLNASGDLNGANNQRIPAGPSGVNQPIPSVTAGGQNQFRPSGGLGNQGSRPTESSNQDELLPTEAEIEAITSDVESNSVAPKATIREILDTLQAKRQNATPKDLFSLAWACYHNGSSRFVNLNTDAPCGITHADLKTLWKASATLRQFCSYYAKSCYVSGKQQKKPPANWSRKGYPEEAKFAGFDFFNAVLSESSPAPPGGMRFKPTQAEILGHSMNAKMSIVESRQSSHMVSTRADLLGRQQINEQPKPPMITF

ORF5

Non-structural protein ORF5

224 amino acids

Seems to play a role in the inhibition of host innate immune repsonse.

25.28 kDa

MAFSASLFKPVQLVPVSPAFHRIESTDSIVFTYIPASGYVAALAVNVCLIPLLLLLRQDTCRRSIIRTMVLYFLVLYNFLLAIVLVNGVHYPTGSCLIAFLVILIILWFVDRIRFCLMLNSYIPLFDMRSHFIRVSTVSSHGMVPVIHTKPLFIRNFDQRCSCSRCFYLHSSTYIECTYISRFSKISLVSVTDFSLNGNVSTVFVPATRDSVPLHIIAPSSLIV

ORF5

Minor capsid protein

216 amino acids

Component that constitutes the tail found at one end of the virion. Together with Hsp70h and p64, encapsidates the 5'-terminal portion of the viral genome. Movement protein that is involved in local cell-cell movement via plamodesmata. At least five viral proteins, CP, CPm, p6, p64 and Hsp70h are essential for cell-cell movement.

24.288 kDa

MLAPEARGDLIHFTENTRDAMETFFNSYDLAEYSEVNPNKLNRKETDELLGVIRERFKSELVITDEDFVKHLAFALIRAANITTSVKVNYVGAYEYTIGGKKFLVKDAWVFPLIKECMKKFNKPNPVRTFCATFEDAYIVIARSLPKLFLNRTIGKRGIPSGYEFLGADFLTATSVCLNDHEKAIVLQASRAAIDRAVSSSVDGKIVSLFDLGRLS

ORF5

Uncharacterized protein ORF5

135 amino acids

Belongs to the plectrovirus ORF5 family.

15.649 kDa

MVKMINLLVVENNNSNWDKIFSFVFDIFLFIFDVIWNTKLPMTNTSIAYFLVFFMVIKLSIYAIHGTSTQYNNLGSTVNNGVSQVYSSTVRKGINVGKNVYQNSNKQQVKKELKRQSIRYQAKNIRSTKFKGDKK

ORF5

Uncharacterized protein ORF5

134 amino acids

Belongs to the plectrovirus ORF5 family.

15.62 kDa

MINLLVENNNSNWDKIFSFVFDIFLFIFDVIWNTKLPMTNTSIAYFLIFFMVIKLSIYAIHGTSTQYNNLGSTVNNGVSQVYSSTVRGVSDTKQGMQKHIKERKQFKINRNKKQLSSLAKQAKTREQGYRRVHK

ORF5

Uncharacterized ORF5 protein

128 amino acids

14.331 kDa

MSDFNDELVIRCGDTVYSPIVCSVLHPTISAKDEIVMHVDVIHSPPKYRGFANSTRHIMPWRMEHNHKTMSSGLHCTDVLAVEKEALPGAIATFFSSLKTPINITFPDKVHVTREPLKPNDNAHIDEA

ORF5

Uncharacterized protein V5

119 amino acids

14.58 kDa

MSNITYEFNENDVIVNNNGMPCIQRPFDKEALYSLLDKMSKEQLDELIEDINDEKRLREFKEEKLKKVRSRMNRELKIYKQELENKKQRMKMLTEQEEEEEEEEEEPPKPKKKVINRKK

ORF5

Uncharacterized protein ORF5

101 amino acids

10.863 kDa

MGGAYITSRYWLVPAHVTSRLRLTSFTGHRGVARVDGPMSSLGLNIIKCAVNGGMDMTGKMSSSWTIFMGGYLIARCSASATVIHIKCQSRALLWSLPARG

ORF5

Protein ORF5

96 amino acids

Plays a central role in the packaging of viral DNA into phage procapsid, which occurs in the late stage of infection. Can interact with the replicative complex after the completion of one round of DNA synthesis. When protein ORF5 is bound to the replicative form, the complex becomes accessible to procapsid and serves as a DNA packaging apparatus (By similarity).

Belongs to the microviridae C protein family.

11.469 kDa

MHMFYYSIYDRKARSYGDLISFPSGEKEAAIRWFRDVVMDSDSKNILHRYPEDFDFCYIGYFDKDKGRFYPVDAGIVTIINAGEFFLDSEYRQEEV

ORF5

RNA silencing suppressor

90 amino acids

Suppressor of viral-induced RNA silencing. The potential mechanism of action is based on sequestering siRNAs.

Belongs to the carlaviruses nucleic acid-binding protein family.

10.528 kDa

MDDPSFLTGRSTFAKRRRARRMNVCKCGAIMHNNKDCKSSSISSHKLDRLRFVKEGRVALTGETPVYRTWVKWVETEYHIYIVETSDDED

ORF5

Protein VP5

84 amino acids

Plays a central role in the packaging of viral DNA into phage procapsid, which occurs in the late stage of infection. The protein VP5 can interact with the replicative complex after the completion of one round of DNA synthesis. When protein VP5 is bound to the replicative form, the complex becomes accessible to procapsid and serves as a DNA packaging apparatus (By similarity).

Belongs to the microviridae C protein family.

9.589 kDa

MQLKVFSIRDSKTGVYGTPFYQHTHGQAERSFQQLAKDPQSTVANHPEDFDLFHLGEYDDQTGKLTPLDTPEHCVKAIDLIKQQ

ORF5

Protein VP5

84 amino acids

Plays a central role in the packaging of viral DNA into phage procapsid, which occurs in the late stage of infection. The protein VP5 can interact with the replicative complex after the completion of one round of DNA synthesis. When protein VP5 is bound to the replicative form, the complex becomes accessible to procapsid and serves as a DNA packaging apparatus (By similarity).

Belongs to the microviridae C protein family.

9.589 kDa

MQLKVFSIRDSKTGVYGTPFYQHTHGQAERSFQQLAKDPQSTVANHPEDFDLFHLGEYDDQTGKLTPLDTPEHCVKAIDLIKQQ

ORF5

Protein ORF5

83 amino acids

Plays a central role in the packaging of viral DNA into phage procapsid, which occurs in the late stage of infection. Can interact with the replicative complex after the completion of one round of DNA synthesis. When protein ORF5 is bound to the replicative form, the complex becomes accessible to procapsid and serves as a DNA packaging apparatus (By similarity).

Belongs to the microviridae C protein family.

9.494 kDa

MDKLISLKDVMRFIFKFGLPGLCIAVGLIVMVANKWLGIPWPCYVIVGSVSLSFGLILFIVQTIKEIIIYRKEKKDENIYSKK

ORF5

Uncharacterized protein p7

59 amino acids

6.684 kDa

MPKVESHHWRRRRILGNRLDGRGVVPPTTHHSGYQWYTTMAGSAKVLCTKNPWKRGGGG

ORF5

Uncharacterized protein 5

57 amino acids

6.117 kDa

MIQSLLLDLLPIRSFVMLALVVVGLQLVGVDVIGPAIDMARNFMLDTFSVSDLGSLV

ORF5

Uncharacterized protein ORF5

15 amino acids

1.891 kDa

MQMQEKGWKIIIEEQ