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OPR3

Gene
OPR3
Protein
12-oxophytodienoate reductase 3
Organism
Solanum lycopersicum
Length
396 amino acids
Function
Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. It is required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program (By similarity). In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively.
Similarity
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.
Mass
43.52 kDa
Sequence
MASSAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITEGTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGAAPISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRKTFYTQDPVVGYTDYPFLQGNGSNGPLSRL

Gene
OPR3
Protein
12-oxophytodienoate reductase 3
Organism
Arabidopsis thaliana
Length
391 amino acids
Function
Specifically cleaves olefinic bonds in cyclic enones (PubMed:11094980). Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules (PubMed:29291349, PubMed:10872231, PubMed:10973494). Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program (PubMed:10899973, PubMed:10973494). In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively (PubMed:10872231). Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT) (PubMed:19605548).
Similarity
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.
Mass
42.691 kDa
Sequence
MTAAQGNSNETLFSSYKMGRFDLSHRVVLAPMTRCRALNGVPNAALAEYYAQRTTPGGFLISEGTMVSPGSAGFPHVPGIYSDEQVEAWKQVVEAVHAKGGFIFCQLWHVGRASHAVYQPNGGSPISSTNKPISENRWRVLLPDGSHVKYPKPRALEASEIPRVVEDYCLSALNAIRAGFDGIEIHGAHGYLIDQFLKDGINDRTDQYGGSIANRCRFLKQVVEGVVSAIGASKVGVRVSPAIDHLDATDSDPLSLGLAVVGMLNKLQGVNGSKLAYLHVTQPRYHAYGQTESGRQGSDEEEAKLMKSLRMAYNGTFMSSGGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYNRKTFYTQDPVVGYTDYPFLAPFSRL

Gene
OPR3
Protein
Putative 12-oxophytodienoate reductase 3
Organism
Oryza sativa subsp. japonica
Length
382 amino acids
Function
Putative oxophytodienoate reductase that may be involved in the biosynthesis or metabolism of oxylipin signaling molecules.
Similarity
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.
Mass
42.409 kDa
Sequence
MVQAQAAAKEAAAAAIPLMAPYKMGRFELSHRVVLAPLTRCRSYDHVPQPHAALYYSQRATNGGLLISEATGVSATGEGYPEIPGVWTRQQVKAWKPIVDAVHRKGALFFCQLAHVGRASTNDQQPNGQAPISSTDKQITPDDSHTVYSKPRRLRTDEIPHVVDDFRVAARNAIEAGFDGVEIHGAHGYLIDQFMKDSANGRTDQYGGSLENRCRFAVEVIDAVVAEVGADRVGIRLSPYIDFMDCFDSNPEALGSYMVRQLNKHPELLYCHMVEPRMATVEGRRKINHGLLPFRKQFNGTFIASGGYDREEGNKVVDDGYADLVAYGRLFLANPDLPRRFELNAPLNKYDGSTFYTHDPVVGYTDYPFLEEKKEDSATVIV