Protein
GDP-fucose protein O-fucosyltransferase 1
Organism
Drosophila melanogaster
Function
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in Notch signaling. Initial fucosylation of Notch/N by POFUT1 generates a substrate for Fringe/Fng, an acetylglucosaminyltransferase that can then extend the fucosylation on the Notch EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by Delta/Dl or Serrate/Ser. Also required for the transport of Notch/N to the early endosome.
Similarity
Belongs to the glycosyltransferase 65 family.
Sequence
MQWLKMKLRFVNLILLLISSTCAQLGGDPNGYLTYCPCMGRFGNQADHFLGSLAFAKALNRTLILPPWVEYRRGELRSRQVPFNTYFEVEPLKEYHRVITMADFMWHLADDIWPESERVSFCYKERYSLQQEKNDPDKPNCHAKDGNPFGPFWDTFHIDFVRSEFYAPLHFDVHHSNEAAKWQTKYPAESYPVLAFTGAPASFPVQLENCKLQRYLQWSQRYREASKDFIREQLPRGAFLGIHLRNGIDWVRACEHVKDSQHLFASPQCLGYKNERGALYPELCMPSKEAIIRQLKRTIKNVRQTQPDNEIKSVFVASDSNHMIGELNTALSRMGISVHKLPEDDPYLDLAILGQSNHFIGNCISSYSAFEKRERDVHGFPSYFWGFPKEKDRKHTNVHEEL