NGLY1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

654 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.

Belongs to the transglutaminase-like superfamily. PNGase family.

74.39 kDa

MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRARKLDKGINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRIIVELVEFISPKTPKPGELGGRISGSVAWRVARGEMGLQRKETLFIPCENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKFECGSVGLKVDSISIRTSSQTFQTGTVEWKLRSDTAQVELTGDNSLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKFSDL

NGLY1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

654 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).

Belongs to the transglutaminase-like superfamily. PNGase family.

74.524 kDa

MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVESSQQPAASTQLPTTPSSNPSGLNQHTRNRQGQSPDPPSASTVTPDSAILGVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEKLSRVRKLDKGTNISDEDFLLLELLHWFKEEFFHWVNNILCSKCGGQTRSRDRSLLPNDDELKWGAKKVEDHYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHEEVIARRTKVKEALLRETINGLNKQRQLFLSENRRKELLQRIIVELVEFISPKTPKPGELGGRISGSVAWRVARGEMGLQRKETSFIPSENEKISKQLHLCYNIVKDRYVRVSNNNQTISGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKFECGSVGLKIDSISIRTTSQTFQTGTIEWKLRSDTARVELTGDNNLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEIIIKFSDL

NGLY1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

651 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).

Belongs to the transglutaminase-like superfamily. PNGase family.

73.903 kDa

MAGSAGLSSSPSSPAVRELCKNARDTFLEASRLLLTYADNILRNPYEEKYRSIRNGNPAFSTRLLPVRGAVECLFEMGFQEGETHMVFPKEASIEQLRKVRDLIAVERSSRLNESNQVHRSASSETVAITQAIAHQPSRPAGSVPTPDHQQPEPSLLQSLKMAADILTTLQSKCDHLILAYESTSLQQKALALIPLQQLKEKAQRKLAQATRLDKGEHVNEEDFLLLELLNWFKNDFFHWVDNLPCSRCGGQTEGKRDYLSPTDDDLRWNADRVENHYCSQCQFCNRFPRYNNPEKLLETRRGRCGEWANCFTLCCRAVGFEARYVRDWTDHVWTEVYSASQKRWLHCDPCENVCDKPLLYEIGWGKKLSYVIAFSKDEVVDVTWRYSCKHQEVLTRRTALSEAKLRETINAINKKKQQSLSEGRRKELLERTIVELAEFISPKTPKPGEFGGRTSGSMAWRIARGETGSEERKEVIFIPSEKEKASKLFHLMYNVVEDSYTRISNNNEQITGWGTGIWKAESIARKVETDWKMVYLARKEGSSSASISWKFECKSVGLKIDNISIRTSSQTFHSGRIKWRLYSPTAEVILVGDNSLSSYSDFCGATEVTLEATLSGGDGKAAWQHTQLFRNSLAGCGENCLEMIIKLADL

Ngly1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

651 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.

Belongs to the transglutaminase-like superfamily. PNGase family.

74.275 kDa

MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL

Ngly1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

651 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).

Belongs to the transglutaminase-like superfamily. PNGase family.

74.677 kDa

MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAVERRSRLDGSSQKVEFSQHPAAVRLPAEQPEDPTGLMQHSGNQPGQPLSLPSAPLVVGDSTIFKVLQSNIQHVQLYENPVLQEKALACIPVNELKRKSQEKLFRARKLDKGTKVSDEDFLLLELLHWFKEEFFHWVNNVVCSRCGRETRSRDEALPPNDDELKWGAKNVEDHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHEEVMSRRTKVKEELLRETINGLNKQRQLLLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETCLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNANISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDNVSIRTSSQSFETGSVRWKLRSEMAQVNLLGDRNLRSYDDFCGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDHAENGLEIIITFSDL

ngly1

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

644 amino acids

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).

Belongs to the transglutaminase-like superfamily. PNGase family.

72.489 kDa

MPGSQGVLALCENTTEVFLDVSKLLITYADNILRNPNEEKYRSIRIGNPTFSTKLLPVKGAVECLFEMGFEEAETHLVFPRSASVEKLRQVRETIAAERDQRLGASNTASSPQNASVPPVPAPPPSLSSTASAGPAAAAVPTPAPASIPFTSSSATFQRTVQSNFQHVLVYEDPELQQKALECIPHELLRSRAKERLKQANDADAACSLGEEDMLVLDLLQWFKSDFFSWVDNLPCIQCGGKTQPSGSLSPSSDDLHWDAGRVENHFCHTCQLSTRFPRYNNPEKLLETRKGRCGEWANCFTLLCRALGLEARYIWDSTDHVWTEVYSQSQRRWIHCDPCENACDKPLLYEVGWGKKLSYILAFSKDQVADVTWRYSCKHPEVLSRRTQVQETWLLHMLNKLNAERQQFLGAERKQQLLQRLLVELVEFISPKSPQPGELGGRMSGSLAWRAARGETGASNAKEDTQENVFTPSESEKESKRFHICYNVTKDCYYRVSNGQETIQGWQKGAWRTENMFRKEEHDWQMVYLARTEGASFGRMSWKFNCAPAGMKMKSASVRVFSQTFHSGTVRWSLSTNETTTEFPGDGELHSFSNVSGGTELIVEAELTGGDGSTSWQHAQLFRQSLKDTEKVLFEVMVEMEKS