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NCS6

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Ustilago maydis (strain 521 / FGSC 9021)
Length
405 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
45.123 kDa
Sequence
MAPKLCVICNAERAVLRRPKTAQQICKECFFYVFETEVHHTIVSNNLFKRGDRVAIGASGGKDSTVLAHCMKVLNERYDYGLDLFLLSIDEGITGYRDDSLETVKRNQQQYQLPLKVLGYGELYGWTMDDIVKSVGRKNNCTFCGVFRRQALDRGAAALGVDHIVTGHNADDVAETVLMNVLRGDIARLERCTDIVTKGPDGSEDDQHQEGCGSSGSNGIGVGGSGIRRSKPFMYAYEKEIVMYAYFKKLDYFSTECIYSPNAYRGYARTFLKDLEAIRPSSIVDIIQSGQNLKVSQVVKQSRKTQLQNCLRCGYISSNTVCKACILLEGLEKGTPQLGIGSEKSRKIKDIRSENSKEVEKVSITADGKERKERRIPQWTGVAKDAPPISSLPFVPPQHKVDLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Candida albicans (strain SC5314 / ATCC MYA-2876)
Length
395 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
44.412 kDa
Sequence
MPESTNTIINSSVKKIKLSALCELCHGRKAVMKRPKNLMKLCKECFYNIFETEIHNTIISNDLFYRGEKIAIGASGGKDSTVLASILKTLNERYDYGLNLVLLSIDEGIKGYRDDSLATVKRNQKQYDMPLEIVSYKDLYNWSMDEIVACAGIRSSCTYCGVLRRQALDRGAEKLGIKHVVTGHNADDVAETVLMNLLRGDVARLESSTNIMTTSAGSPIKRSKPFKYTYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELLKSLESIRPSCIMDIIYSGEHLVLAPKKQKRKTVAYKNKNKNKKKSNSEQEEQEKQEQEVNPDGSISLNRNGIKKDGNTCEKCGYLSSNKICKACMLLNGLEINRAKVTIDNNSAIDGAAKLTKKLEQLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)
Length
379 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
42.563 kDa
Sequence
MVESSNTKKVKVSSLCELCHGRKAVMKRPKNLRKLCKECFFHVFETEVHNTIVENKLFANVENSEFPERRAVAIGASGGKDSTVLASVMKTLNERYNYGLKLVLLCIDEGIKGYRDHSLETVKMNQKEYDMPLEILSYKDLYDWTMDEVVSCAGIRSSCTYCGVLRRQALDKGAEKLGINHIVTGHNADDMAETVLLNLLRGDINRIENSTKIITDSENSVIQRSKPFAYMSQKEIVLYAHYKNLTYFSTECTYSEEAFRGECRSLFHSLSAVLPSVHTNTIYSGQQFKRKAKAMKRQNNKNNKNNKNNMADEHEVLPNGTVAIKESKRCKKCGSLASNDLCQACFLLAGLEVSRAKVSIDSEGDGAAKLSKTLEGLTF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
Length
376 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
41.984 kDa
Sequence
MTEISAKKVKVSALCELCHARKAVMKRPKNLQKLCKDCFYKVFETEIHNTIVDAKLFSPGDKVAIGASGGKDSTVLASVLKTLNERYDYGLILVLLSIDEGIKGYRDDSLATVKRNQVQYEMPLEIISYRDLYNWTMDEIVSCAGIRSSCTYCGVLRRQALDRGAAKLGINHVVTGHNADDMAETVLMNLLRGDTARLEKSCAIITQSAGSPIKRSKPFKYTYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELLKSLESIRPSCIMDIIYSGEHLVLAPKKKKITTSYKTNKKKTHTENEVNADGSVSLGRKKQFEDGNRCEKCGYLSSNRICKACMLLAGLEMNRAKVSIDNNTAVDGAAVLTRTLEQLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Length
373 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
41.997 kDa
Sequence
MYIPPSDPRRPAKVTAGQLCELCHARKALVKRPKNLQKVCKLCFFHVFETEIHNTIMENKLFQRGERVAVGASGGKDSTVLAYILKLLNERHDYGLEIVLLSIDEGIVGYRDDSLATVKRNQEQYGLPLKIVSYKDLYDWTMDEIVACAGMRNSCTYCGVFRRQALDRGAAMLDIHHVVTGHNADDMAETVLMNILRGDVARLEKSTAILTQSSGSPVKRSKPFKYAYQKEIVLYAHYKKLDYFSTECSYAPEAFRGTARELMKNLEAVRPSCIIDIIHSGEALRLRPRPKKRAPPPGSVEIRADGSASLFRNEGFVDGNRCERCGYLSSNRICKACMLLEGLEKNRARVQIADDTSTEGAARLTRTLEKLHF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Malassezia globosa (strain ATCC MYA-4612 / CBS 7966)
Length
373 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
41.472 kDa
Sequence
MPRECELCGTERAVLRRPKTGDLVCRACFFHVFETEVHQTIVEAQLFRRGDRVAIGASGGKDSTVLAYVMKTLNERYDYGLNLFLLSIDEGITGYRDDSLETVKRNQEQYGIPLKVLGYKELYGWSMDDIVASIGRKNNCTFCGVFRRQALDRGAASLGVDHIVTGHNADDMAETVLMNVLRGDIARLERCTDIITKGPDGVDGDEDDDETEGCGAGRSGFGGSGIRRSKPFKYAYEKEIVMYAYFKQLDYFSTECIYSPNAYRGYARAFLKDLESIRPSSIIDIIHSGENLHVAGQVKRAVQRTCTRCGYISSNELCKACVLLEGLNRGAPALGVRSDKSRAVREARLDGTSDIGRTIPRWEGVQRSSATAW

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)
Length
369 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
41.532 kDa
Sequence
MEEKKIRKVTLCDICHQSKAVMKRPKNLQKLCKECFYHVFETEIHNTIVDAQLFKPGDKVAIGASGGKDSTVLASVLKTLNEKYNYGLTLVLLSIDEGIVGYRDDSLATVKRNQIQYEMPLEIISYKDLYNWSMDEIVSCAGIRSSCTYCGVLRRQALDRGAAKLEIDHVVTGHNADDMAETVLMNLLRGDIARLERSCSILTSSEGSPIKRSKPFKYTYQKEIVLYAHYKKLDYFSTECSYAPEAFRGTARELLKALESVRPSCIMDIIYSGEHLVVAHKKKRTTEKYKNKPKKNIETEVNSDGSVNLTKKTDGSRCEKCGYLSSNKICKACVLLAGLEMNRPKVNIENNTAVEGSAKVMKTLEQLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Yarrowia lipolytica (strain CLIB 122 / E 150)
Length
365 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.376 kDa
Sequence
MLCVLCECRKAMLKRPKTLQPICKPCFYNVFETEIHNTIVESNLFFPGERVAIGASGGKDSTVLAHVMKTLNERYNYGVDFVLLSIDEGIHGYRDDSLETVKRNKVQYDMDLEVVSYSELYGWSMDQIVAQIGNKNNCTYCGVFRRQALDRGSAKLGIAHIVTGHNADDMAETVLMNLLRGDTARLDRCTELVTGSDDSPVKRSKPLKYAYEKEIVLYAHYKKLDYFSTECTYSPEAFRGTARTLIKNLEAIRPSTIIDIIHSGEAFVLKKKKEKKGKGSVVVKAKVEEKPVPSGGCSVPLAFDSSLGLSNRCTKCGYLSHNAVCKACVLLEGLNAGRAKMQIEGDSADGAAKNIKTLEKLALSI

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Oryza sativa subsp. japonica
Length
363 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.873 kDa
Sequence
MDSAVDGPRQPPARAGSRLCTRCGERKAALKRPKTLEQICRECFYVVFEDEIHQTIVDNNLFKPGDRVAIGASGGKDSTVLAYVLSELNRRHKYCLDLFLLSVDEGITGYRDDSLETVKRNEIQYGLPLKIVSYKDLYGWTMDDIVKAIGLKNNCTFCGVFRRQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERMRPRAILDIIKSGENFRISTTTRMPEQGTCERCGYISSQKLCKACVLLDGLNRGLPKLGIGRTKGIAGGDGDCEQQATRSERNRSSLQGKHGNFDF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294)
Length
363 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
41.325 kDa
Sequence
MFTAPSDPVNVRKELKVTQLCELCHSRKAIIRRPKNLQKICKLCFFHVFETEIHETIVSNDLFYRGERVAVGASGGKDSTVLAYVLKLLNERHDYGIEIVLLSIDEGIVGYRDDSLATVKRNQQQYQLPLEIFSYQDLYDWTMDDIVETAGIRNSCTYCGVLRRQALDRGAAKLEIKHVVTGHNADDMAETVLMNILRGDVARLEKSTSIMTSSTGSPIKRSKPFKYCYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELMKNFEAIRPSCIIDIIASGESFVLKQKMGKQNKKKIITYNDGNRCERCGYLSSNKICKACMLLEGLEKSRAQIKIQVQVPGEPQQEGPAKVLRTLERLTF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Saccharomyces cerevisiae (strain RM11-1a)
Length
359 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.086 kDa
Sequence
MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQCFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVLLSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYCGVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGSPIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCIIDIIQSGENLALKAKKSNARKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRAQVAIENDTSADGAALKLRALEKLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Saccharomyces cerevisiae (strain YJM789)
Length
359 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.086 kDa
Sequence
MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQCFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVLLSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYCGVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGSPIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCIIDIIQSGENLALKAKKSNARKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRAQVAIENDTSADGAALKLRALEKLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Length
359 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. May also be involved in protein urmylation and in invasive and pseudohyphal growth.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
39.987 kDa
Sequence
MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQCFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVLLSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYCGVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGSPIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCIIDIIQSGENLALKAKKSNAGKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRAQVAIENDTSADGAALKLRALEKLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)
Length
358 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.578 kDa
Sequence
MSYTAPTDPVNKQEVVKLSQLCELCHGRKAVLRRPKNLQKICKECFYYVFETEIHNTIVSNNLFQRGEKVAIGASGGKDSTVLAHILKLLNDRHDYGVKLVLLSIDEGIVGYRDDSLATVKRNQKQYQLPLEIVSFRDLYDWTMDEIVAIAGIRNSCTYCGVFRRQALDRGAAKLDINHVVTGHNADDMAETVLMNILRGDVARLERSTAIITQSAGSPIRRSKPFKYCYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELMKNLEAVRPSCIIDIIQSGENLVLKKKKRHPNSKVEFKDGNRCERCGYLSSNKICKACMLLQGLEKNRASMSIDNDTNVDGAARVMRTLEKLSF

Gene
NCS6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Arabidopsis thaliana
Length
355 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
40.279 kDa
Sequence
MEAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGERVAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGLPLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPGSVAKSIESKQCGSLDF

Gene
ncs6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Length
341 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
37.956 kDa
Sequence
MPPSPCARCHDQRAVIIRPKNRQKLCRTCFLHVFETEVHETITSTSLFHPGERVAIGASGGKDSTVLAAVLKTLNERYNYGLDLCLLSIDEGIKGYRDDSLETVKRNAQQYNMPLVIVSYGDLYGWTMDQVVAQVGKKGNCTYCGVFRRQALDRGAAKLGIKHVVTGHNADDVAETVMMNLLRGDLPRLSRGTSIVTGSAASDIKRSKPLKYAYEKEIVLYAHHRQLDYFSTECIYSPEAFRGSARTLIKDLEKIRPSSILDIVKSGEDMALLKKVKSQVLGTCERCGYISSQRVCKACTLLEGLNKNRPKTAIEMEIGLEDEESSSTLRRQMEKVELGGV

Gene
ncs6
Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Length
335 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Mass
37.861 kDa
Sequence
MSNKLCQLCNERRPALVRPKTGQKICKECFYYVFETEIHNVIIENKLFVRGERVGIGASGGKDSTVLAYVMKLLNERYDYGLELYLISVDEGIRGYRDDSLDTVKRNQQQYGLPMKIVSYADLYDGWTMDNVVARIGTKNNCTYCGVFRRQALDRAALSLDIHHLVTGHNADDIAETILMNLLRGDVARLPRSTEITTQSDSSPTKRSKPFKYSYEKEIVLYAHYKKLDYFSTECTYSPEAFRGTARAMIKQLENIRPSSILDIIYSGESMQLASSVQEQLPQQTTCERCGFISSNRICKACMLLEGLNKGITGLGLGSDRKTKKLQSQIPACAE