Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.
Similarity
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.
Sequence
MPDIMPDDGLVLGLEGTAWNLSAALFGDDLVALHSSPYVPPKGGIHPREAAQHHASAMKEVVSRVLTEPERIRAVAFSQGPGLGPSLRTVATAARALSIALDVPLVGVNHCVAHVEIGRWATGFSDPIVLYASGANTQVLGYLNGRYRIFGETLDIGLGNGLDKFARSHDLPHPGGPAIERLAREGNYIELPYTVKGMDLAFSGLVSAAQESSAPLEDVCFGLQETAFAMCVEVTERALAHAGKDEVLLVGGVGANGRLQEMLRVMCEERGAAFAVPERTFLGDNGAMIAYTGKIMLEHGVVLPLDQSQIRPGYRADEVEVAWRTEPGEVFSIGPHEGGVARGAEAVVEIGEGNVIKRRTGKRYRYPALDRRLIAERTRAEARLIATARRAGVPTPVIRDITADTIVMERIKGEVLKYVTAPETIRLAGEAVGRLHGTGIVHGDLTTSNMIVRDGQCVLIDFGLASTSSEVESRGVDLHVFFQTLESTTENFQELKEAFVEGYTAVFPGAGEVLAREHEVELRGRYL