mtr1

Ribosome quality control complex subunit 2

1021 amino acids

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mtr1/rqc2 is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. Mtr1/rqc2 is important for the stable association of rkr1/ltn1 to the complex. Mtr1/rqc2 recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal Ala and Thr extensions (CAT tails). CAT tails induce a heat shock response.

Belongs to the NEMF family.

114.24 kDa

MKQRFSALDIAAIAAELREQVVGCRLNNFYDLNARTFLLKFGKQDAKYSIVIESGFRAHLTKFDRENAPLSGFVTKLRKHIKSRRLTGVSQLGTDRVLVFTFGGGANDQDPDWTYYLVCEFFAAGNVLLLDGHYKILSLLRVVTFDKDQVYAVGQKYNLDKNNLVNDNKSQSTIPHMTAERLNILLDEISTAYASPTSINEPLPDQQLSSSTKPIKVPKPVSLRKALTIRLGEYGNALIEHCLRRSKLDPLFPACQLCADETKKNDLLAAFQEADSILAAVNKPPVKGYIFSLEQALTNAADPQHPEECTTLYEDFHPFQPLQLVQANRKCMEFPTYNECVDEFFSSIEAQKLKKRAHDRLATAERRLESAKEDQARKLQSLQDAQATCALRAQAIEMNPELVEAIISYINSLLNQGMDWLDIEKLIQSQKRRSPVAAAIQIPLKLIKNAVTVFLPNPESVDNSDESSETSDDDLDDSDDDNKVKEGKVSSKFIAVELDLSLGAFANARKQYELRREALIKETKTAEAASKALKSTQRKIEQDLKRSTTADTQRILLGRKTFFFEKFHWFISSEGYLVLGGRDAQQNELLFQKYCNTGDIFVCADLPKSSIIIVKNKNPHDPIPPNTLQQAGSLALASSKAWDSKTVISAWWVRIDEVSKLAPTGEILPTGSFAIRAKKNYLPPTVLIMGYGILWQLDEKSSERRKARRLEMEVVETQGKVSELKMEGTSVTSEDNIQDVVSEVSYNEDTNNQSTPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIEDATDPQTILAILKQKKAKKKHAAREMEISSQIPSNDSSNVQTPTAESEIEEDGVSEPISAEVIEDQSRNSEAENEKGLSTEQRDEKKHAKVESFQRQEMPRSLFEEIFFAIDSLTPNPQQQDTVINAVPTFAPYNAMTKFNQKVKVMPGTGKVGKAARESIAYFMKKLPKSSKEAAYLENLKDGEIVAPISVSRLKMVFGSSGNTKKSKK

MTR1

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

370 amino acids

S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of spliced leader and U1 small nuclear RNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped RNA to produce m(7)GpppNmp (cap1). Cap1 modification is linked to higher levels of translation. Recognizes a guanosine cap on RNA independent of its N(7) methylation status.

40.896 kDa

MPAVADCTVVFPLRHDPASTHPDVSGLVGKAFERVNWRDAFDTFLAEERSALWAAKTAFDNTDTSAYIAARDALFPQAVSGVHGAVAFRNRAGHKLHETMEAVGLWEYLKGGATRAKGTFTFVDVCGGPGAFSQALFAMGKEHKLRLRGFGLTLRNVKGLDWYTDLPSRSFFPCYGIDGTGDVFKLENIESLCSLTCKENVRLVVADGGFDVPTEVVNFQETISCRIVYGQWLSAVKLLRPGGCFVLKLFDCFSPFTRAILFLTTHLYESVQVVKPRHSRVVNSERYLVCIGFIGAPKQWLEHFERCYQEGFVDNDNIPTVLPTSLFSGDKIFGADVERMSATIASNQVSGLHAILEKLQSKPAMEEVKS