Protein
Glutamate formimidoyltransferase
Organism
Streptococcus pyogenes serotype M1
Function
Catalyzes the transfer of the formyl group from N-formylglutamate to tetrahydrofolate (THF) to yield 5-formyltetrahydrofolate (5-CHO-THF) and glutamate (Glu). The triglutamate form of 5-CHO-THF (5-CHO-THF-Glu3) can also be used as substrate. It can also catalyzes the transfer of the formimino group from N-formiminoglutamate to tetrahydrofolate (THF) to yield 5-formiminotetrahydrofolate (5-NH=CH-THF) and glutamate (Glu). It can replace YgfA to catalyzes the irreversible ATP-dependent transformation of 5-CHO-THF to form 5,10-methenyltetrahydrofolate (5,10-CH=THF).
Similarity
Belongs to the formiminotransferase family.
Sequence
MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASHNRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAPDYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIGVMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDVAEYYLQVEDFDYHKQILENHLLG