Protein
2-isopropylmalate synthase 2, mitochondrial
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Redundant to LEU4, responsible of about 20% of alpha-IPMS activity. Involved in leucine synthesis.
Similarity
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.
Sequence
MVKHSFIALAEHASKLRRSIPPVKLTYKNMLRDPSVKYRAFAPPKMVKRIWPDKTIQKAPRWLSTDLRDGNQSLPDPMSVAQKKEYFHKLINIGFKEIEVSFPSASQTDFDFTRYAVENAPDDVGIQCLVQSREHLIKRTVEALTGAKRATIHTYLATSDMFREIVFNMSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFSTHITEREKVCISTHCHNDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNLDFSDLTSISEIVHRCNKIPIPPRAPYGGELVVSAFSGSHQDAIKKGFAIQNKKQAQGETRWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVIMRSLGLDVPRPMQVDFSNTLQKNADALGRELKSEEITKLFKETYNYNNNEHIYVTLLNYEVKKLNPERRALVGQVEINDKVVNIEGYGNGPISSLVDALSNLLNVKLSVQNYSEHSLGSGSATQAASFINLSYIKDINNHATSNMWGVGVSEDTGDASIKAVFATVNNIIHSGDVLLAE