HEN1

Small RNA 2'-O-methyltransferase

942 amino acids

Methyltransferase that adds a methyl group to the ribose of the last nucleotide of small RNAs (sRNAs). This protects the 3'-end of sRNAs from uridylation activity and subsequent degradation. Can methylate 3'-end of microRNAs (miRNAs), small interfering RNAs (siRNas) and trans-acting small interfering RNAs (ta-siRNAs). Involved in plant development through its role in small RNAs processing. Required for the specification of reproductive organ identities and the probable repression of A class genes. May control floral determinacy possibly by regulating the expression of the C class floral homeotic gene AGAMOUS (AG).

Belongs to the methyltransferase superfamily. HEN1 family.

104.454 kDa

MAGGGKHTPTPKAIIHQKFGAKASYTVEEVHDSSQSGCLGLAIPQKGPCLYRCHLQLPEFSVVSNVFKKKKDSEQSAAELALDKLGIRPQNDDLTVDEARDEIVGRIKYIFSDEFLSAEHPLGAHLRAALRRDGERCGSVPVSVIATVDAKINSRCKIINPSVESDPFLAISYVMKAAAKLADYIVASPHGLRRKNAYPSEIVEALATHVSDSLHSREVAAVYIPCIDEEVVELDTLYISSNRHYLDSIAERLGLKDGNQVMISRMFGKASCGSECRLYSEIPKKYLDNSSDASGTSNEDSSHIVKSRNARASYICGQDIHGDAILASVGYRWKSDDLDYDDVTVNSFYRICCGMSPNGIYKISRQAVIAAQLPFAFTTKSNWRGPLPREILGLFCHQHRLAEPILSSSTAPVKSLSDIFRSHKKLKVSGVDDANENLSRQKEDTPGLGHGFRCEVKIFTKSQDLVLECSPRKFYEKENDAIQNASLKALLWFSKFFADLDVDGEQSCDTDDDQDTKSSSPNVFAAPPILQKEHSSESKNTNVLSAEKRVQSITNGSVVSICYSLSLAVDPEYSSDGESPREDNESNEEMESEYSANCESSVELIESNEEIEFEVGTGSMNPHIESEVTQMTVGEYASFRMTPPDAAEALILAVGSDTVRIRSLLSERPCLNYNILLLGVKGPSEERMEAAFFKPPLSKQRVEYALKHIRESSASTLVDFGCGSGSLLDSLLDYPTSLQTIIGVDISPKGLARAAKMLHVKLNKEACNVKSATLYDGSILEFDSRLHDVDIGTCLEVIEHMEEDQACEFGEKVLSLFHPKLLIVSTPNYEFNTILQRSTPETQEENNSEPQLPKFRNHDHKFEWTREQFNQWASKLGKRHNYSVEFSGVGGSGEVEPGFASQIAIFRREASSVENVAESSMQPYKVIWEWKKEDVEKKKTDL

HEN1

Small RNA 2'-O-methyltransferase

423 amino acids

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Required for programmed DNA elimination.

Belongs to the methyltransferase superfamily. HEN1 family.

49.946 kDa

MIEAYETDVFMDPIGMKVWEKRHQYVATKLSALNCKRVLDMGTNTCKLIQRLSRSLQFTQIDGLDIDGQLLETQGIQNAKPDLIQNQYASMRDHQLVVNLYQGSALNKIQHLKDQQYDAVILVELIEHLQVEDVFLIEQNLFGFLRPQFVIVTTPNSDFNVYFNFKEQGVLFRDKDHKFEWSQNQFQIWAQKVCQNYGYKVIELTGVGEHKTEGTKNGFCTQIVVFEKDTQQEKYLNFAFFNLQEGEIRQVCQILYPFESKEQHFQREVVDSIRYILHITDKQNQFEDGSYQNYTTLSRIMQNHSISSNWQIQGDYFKLKTYIQNISEFLVHENQFNFQESFVTLNYQAQMEDEENEDQLESDSENVKMQQQQYYFSNDNCFSTKDTTYSSFSTADNLFSQKIQLGQQQMALEEIELEDTIDY

Hen1

Small RNA 2'-O-methyltransferase

391 amino acids

Methyltransferase that adds a 2'-O-methyl group at the 3'-end of selected small RNAs. Mediates 2'-O-methylation of piRNAs, single-stranded siRNAs and long hairpin RNA genes (hpRNAs). Methylation protects the 3'-end of small RNAs from tailing and trimming and could constitute a recognition signal for appropriate argonaute machineries.

Belongs to the methyltransferase superfamily. HEN1 family.

45.57 kDa

MFSHKFICGSLTKMTETGITFDPPVYEQRYCATIQILEDARWKDQIKKVVEFGCAEMRFFQLMRRIETIEHIGLVDIDKSLLMRNLTSVNPLVSDYIRSRASPLKVQILQGNVADSSEELRDTDAVIAIELIEHVYDDVLAKIPVNIFGFMQPKLVVFSTPNSDFNVIFTRFNPLLPNGFRHEDHKFEWSRDEFKNWCLGIVEKYPNYMFSLTGVGNPPKEYESVGPVSQIAIFVRKDMLEMQLVNPLVSKPNIDKESIPYKLIHTVEYPFYVDTRTEKEKLWTEVQIELQRFKRQFESSEIEEGTYQDTCNMPIAFLLDRLEHVGATKERIEELLLENNLTVENECVLIVSSDQESEWSDPYKFSDRSSQDDALVDQEQEEERWDQGPES