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GA24966

Gene
GA24966
Protein
Adenylyltransferase and sulfurtransferase MOCS3-1
Organism
Drosophila pseudoobscura pseudoobscura
Length
451 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Similarity
In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.
Mass
50.1 kDa
Sequence
MIDSEALESERVKLKRDIADLRANLNRKEQCLRELEAAIAAGEDSDEAEESSNDMPTPQTKLTNDDIARYSRQLILQDFGVQGQLKLKNSSVLIVGMGGLGCPAAQYLVAAGCGHLGLIDYDEVERSNLHRQILHSEHRCGMSKAESARIALLELNSHCQIRCHSRLINSMNAMHIIRPYDVVLDCSDNVATRYLLNDACVMLRKPLVSGSALKMDGQLTVYGYGQGPCYRCIYPVPPPPEAVTNCGDGGVLGAVTGIIGAMQALEAIKLIIGLGDVMSGRLLIFDGSSFMFRNIRIRTKRPNCHVCSAQPLITELIDYEMFCGMHATDKDNPLDLLEPDQRLEVKEYHQKLQSQPHLLLDVRPPAEFEICQLPRSINVPLSEILDDSYLKRFAKQLEDKELPIVLLCRRGNDSQIAVQHITNRFPAHSIRDLVGGLHAWTGSVDATFPIY

Gene
GA24966
Protein
Adenylyltransferase and sulfurtransferase MOCS3-1
Organism
Drosophila pseudoobscura pseudoobscura
Length
451 amino acids
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Similarity
In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.
Mass
50.1 kDa
Sequence
MIDSEALESERVKLKRDIADLRANLNRKEQCLRELEAAIAAGEDSDEAEESSNDMPTPQTKLTNDDIARYSRQLILQDFGVQGQLKLKNSSVLIVGMGGLGCPAAQYLVAAGCGHLGLIDYDEVERSNLHRQILHSEHRCGMSKAESARIALLELNSHCQIRCHSRLINSMNAMHIIRPYDVVLDCSDNVATRYLLNDACVMLRKPLVSGSALKMDGQLTVYGYGQGPCYRCIYPVPPPPEAVTNCGDGGVLGAVTGIIGAMQALEAIKLIIGLGDVMSGRLLIFDGSSFMFRNIRIRTKRPNCHVCSAQPLITELIDYEMFCGMHATDKDNPLDLLEPDQRLEVKEYHQKLQSQPHLLLDVRPPAEFEICQLPRSINVPLSEILDDSYLKRFAKQLEDKELPIVLLCRRGNDSQIAVQHITNRFPAHSIRDLVGGLHAWTGSVDATFPIY