ESYN1

Enniatin synthetase

3132 amino acids

Nonribosomal peptide synthetase that synthesizes enniatin by coupling three D-hydroxycarboxylic acids and three L-amino acids via amide and ester bonds in an alternating fashion (PubMed:25398283, PubMed:28955446, PubMed:28946884). Whereas ESYN1 can accept different amino acids as precursors (L-valine, L-isoleucine or L-leucine), only one species of D-hydroxycarboxylic acid can be found in natural enniatin isolates (D-hydroxyisovaleric acid, D-Hiv) (PubMed:25398283, PubMed:28955446). D-Hiv stems from L-valine deanimation by a valine aminotransferase to 2-keto-isovaleric acid (2-Kiv), which becomes subsequently reduced by a keto-isovaleric acid reductase (KivR) to D-Hiv (PubMed:25398283, PubMed:28955446, PubMed:28946884).

Belongs to the NRP synthase family.

346.593 kDa

MSLHTPSDGQQDPALASKTLCEQISRALGLGQDKIENIFPGTPFQRDVIDCAADDKQRAVGHAVFEIPKDIDAARLAAAWKETVLHTPALRTCTFTSKSGDVLQVVLRDSFVFSWMSGPSVDLKEAVVQDEAAAALAGPRCNRFVLLEDPDTKERQLIWTFSHALVDSTFQERILRRVLKAYKDANDEHPRQFETPDSSQATPEEDLQPNPSKMLKIPQAADMDRAVEFWKDHLSGLNASAFPHLSSHLSMPHPDAKAEHRISYSSSAQQKMSSATICRTALAILLSRYTHSPEALFGIVTEQTPLLEEQLMLDGPTRTVVPIRVSCASEQSVSDIMSTIDSYDQTMRQFAHAGLRNIASAGDDESAACGFQTVLLVSDGDAQPASTWEILKKTEEPEGFIPCTNRALLLSCQMTSSGAHLTARYDQSIIDAEQMARLLRQLGHLIQNLQTSTDLPVEKVDMMTQEDWLEIERWNSDSIDAQDTLIHSEMLKWTSQSPNKAAVAAWDGEWTYAELDNVSSRLAQHINSIDLGKEHAIVPIYFEKSKWVVASMLAVLKAGHAFTLIDPSDPPARTAQVVQQTSATVALTSKLHRETVQSTVGRCIVVDEEFVKSLPQSSELSASVKAHDLAYVIFTSGSTGIPKGIMIEHRSFSSCAIKFGPALGITSDTRALQFGSHAFGACILEIMTTLIHGGCVCIPSDDDRMNNVLEFINRTNVNWVMATPSYMGTFQPEVVPGLKTLVLVGEQMSASVNEVWAPRVQLLNGYGQSESSSICCVAKISPGSSEPNNIGHAVGAHSWIVDPEDPNRLAPIGAVGELVIESAGIARDYIVAPTQDKSPFIKTAPTWYPAKQLPDGFKIYRTGDLACYASDGSIVCLGRMDSQVKIRGQRVELGAVETHLRQQMPDDMTIVVEAVKFSDSSSTTVLTAFLIGAGEKNSHILDQRATREINAKMEQVLPRHSIPAFYISMNNLPQTATGKVDRRKLRIMGSKILSQKTHSTPSQQSQAAISSGTDTETKLESIWITSLDLEPGSANMSATFFEMGGNSIIAIKMVNMARSNGIELKVSDIYQNPTLAGLKAIVIGTSLPYSLIPKVTRQGPVSEQSYAQNRMWFLDQLSEGASWYLIPFAVRMRGPVDVDALTRALLALEQRHETLRTTFENQDGVGVQIIHDRLSKELQVIDALDGDEGGLKTLYKVETTTFDITSEAGWSSTLIRLGKDDHILSIVMHHIISDGWSIDVLRRELIQLYAAALQGKDPSSALTPLPIQYSDFAVWQKQEAQAAEHERQLQYWKKQLADSSPAKIPTDFPRPDLLSGDAGVVPVAIDGELYQKLRGFCNKHNSTAFSILLAAFRAAHYRLTAVDDAVIGIPIANRNRWELENMIGFFVNTQCMRIAVDETDTFESLVRQVRSTTTAAFAHEDVPFERVVSALQPGHRDLSRTPLAQIMFAVHSQKDLGRFELEGIQSEPIASKAYTRFDVEFHLFQQADGLKGSCNFATDLFKPETIQNVVSVFFQILRHGLDQPETCISVLPLTDGVEELRRLDLLEIKRTNYPRDSSVVDVFREQAAANPEVIAVTDSSSRLTYAELDNKSELLSRWLRRRNLTPETLVSVLAPRSCETIVAYVGILKANLAYLPLDVRSPVTRMKDILSSVSGNTIVLMGSGVEDPGFDLPQLELVRITDTFDETIEDVQDSPQPSATSLAYVVFTSGSTGKPKGVMIEHRAIVRLVKSDNFPGFPSPARMSNVFNPAFDGAIWEINWMLLNGGTVVCIDYLTTLDGKELAAVFAKERVNAAFFAPAMLKLYLVDAREALKNLDFLIVGGERFDTKEAVEAMPLVRGKIANIYGPTEAGIISTCYNIPKDEAYTNGVPIGGSIYNSGAYVMDPNQQLVGLGVMGELVVTGDGVGRGYTNPELNKNRFIDITIEGKTFKAYRTGDRMRARVGDGLLEFFGRMDNQFKIRGNRIEAGEVESAMLSLKNVLNAAIVVRGGGEDEGPLEMVGFIVADDKNDTTEEEETGNQVEGWQDHFESGMYSDISTAVDQSAIGNDFKGWTSMYDGKDIDKGEMQEWLDDAIHTLHNGQIPRDVLEIGTGSGMILFNLNPGLNSYVGLDPSKSAVEFVNRAVESSPKFAGKAKVHVGMATDVNKLGEVHPDLVVFNSVVQYFPTPEYLAEVIDGLIAIPSVKRIFLGDIRSYATNGHFLAARAIHTLGTNNNATKDRVRQKIQELEDREEEFLVEPAFFTTLKERRPDVVKHVEIIPKNMKATNELSAYRYTAVVHLRDETDEPVYHIEKDSWVDFEAKQMDKTALLDHLRLSKDAMSVAVSNITYAHTAFERRIVESLDEDSKDDTKGTLDGAAWLSAVRSEAENRASLTVPDILEIAKEAGFRVEVSAARQWSQSGALDAVFHHFPPSSTDRTLIQFPTDNELRSSLTLANRPLQKLQRRRAALQVREKLQTLVPSYMVPPNIVVLDTMPLNTNGKIDRKELTRRARTLPKQQTAAPVPDFPISDIEITLCEEATEVFGMKVEISDHFFQLGGHSLLATKLISRIQHRLHVRVTVKDVFDSPVFADLAVIIRQGLAMQNPVAEGQDKQGWSSRVAPRTEVEKMLCEEFAAGLGVPVGITDNFFDLGGHSLMATKLAVRIGRRLDTAITVKDIFDYPVLFQLAKKLESSHSKSYEESGDDIQMADYTAFQLLDLEDPQDFVQSQIRPQLDSCYGTIQDVYPSTQMQKAFLFDPTTGEPRGLVPFYIDFPSNADAETLTKAIGALVDKLDMFRTVFLEAAGDLYQVVVEHLNLPIETIETEKNVNTATGDYLDVHGKDPVRLGHPCIQFAILKTASSVRVLLRMSHALYDGLSFEYIVRGLHVLYSGRNLPPPTQFARYMQYAAHSREEGYPFWREVLQNAPMTVLHDTNNGMSEQEMPASKAVHLSEVVNVPAQAIRNSTNTQATVFNTACALVLAKESGSQDVVFGRIVSGRQGLPVVWQDIIGPCTNAVPVHARVDDGNPQRIIRDLRDQYLRTLPFESLGFEEIKRNCTDWPEELTNFSVCVTYHNFEYHPESEVDNQKVEMGVLAKYVELSENEPLYDLAIAGEVEADGVNLKVTVVAKARLYNEARIRHVLEEVCKTFNGLNEAL

ESYN1

Enniatin synthase

3131 amino acids

Nonribosomal peptide synthetase that synthesizes enniatin by coupling three D-hydroxycarboxylic acids and three L-amino acids via amide and ester bonds in an alternating fashion (PubMed:7601090, PubMed:10887181). Whereas ESYN1 can accept different amino acids as precursors (L -valine, L-isoleucine or L-leucine), only one species of D-hydroxycarboxylic acid can be found in natural enniatin isolates (D-hydroxyisovaleric acid, D-Hiv) (PubMed:7601090, PubMed:10887181). D-Hiv stems from L-valine deanimation by a valine aminotransferase to 2-keto-isovaleric acid (2-Kiv), which becomes subsequently reduced by a keto-isovaleric acid reductase (KivR) to D-Hiv (PubMed:7601090, PubMed:10887181). Peptide bond formation and N-methylation of the amino acid occur before three enzyme-bound dipeptidols are condensed to a hexapeptidol (PubMed:7601090, PubMed:10887181).

Belongs to the ATP-dependent AMP-binding enzyme family.

346.499 kDa

MSLHTPSDGQQDPALASKTLCEQISRALGLGQDKIENIFPGTPFQRDVIDCAADDKQRAVGHAVFEIPKDIDAARLAAAWKETVLHTPALRTCTFTSKSGDVLQVVLRDSFVFSWMSGPSVDLKEAVVQDEAAAALAGPRCNRFVLLEDPDTKERQLIWTFSHALVDSTFQERILRRVLKAYKDANDEHPRQFETPDSSQATPEEDLQPNPSKMLKIPQAADMDRAVEFWKDHLSGLKCFCLPAFVLSSVYAHPDAKAEHRISYSSSAQQKMSSATICRTALAILLSRYTHSPEALFGIVTEQTPLLEEQLMLDGPTRTVVPIRVSCASEQSVSDIMSTIDSYDQTMRQFAHAGLRNIASAGDDESAACGFQTVLLVSDGDAQPASTWEILKKTEEPEGFIPCTNRALLLSCQMTSSGAHLTARYDQSIIDAEQMARLLRQLGHLIQNLQTSTDLPVEKVDMMTQEDWLEIERWNSDSIDAQDTLIHSEMLKWTSQSPNKAAVAAWDGEWTYAELDNVSSRLAQHINSIDLGKEHAIVPIYFEKSKWVVASMLAVLKAGHAFTLIDPSDPPARTAQVVQQTSATVALTSKLHRETVQSTVGRCIVVDEEFVKSLPQSSELSASVKAHDLAYVIFTSGSTGIPKGIMIEHRSFSSCAIKFGPALGITSDTRALQFGSHAFGACILEIMTTLIHGGCVCIPSDDDRMNNVLEFINRTNVQLGHATPSYMGTFQPEVVPGLKTLVLVGEQMSASVNEVWAPRVQLLNGYGQSESSSICCVAKISPGSSEPNNIGHAVGAHSWIVDPEDPNRLAPIGAVGELVIESAGIARDYIVAPTQDKSPFIKTAPTWYPAKQLPDGFKIYRTGDLACYASDGSIVCLGRMDSQVKIRGQRVELGAVETHLRQQMPDDMTIVVEAVKFSDSSSTTVLTAFLIGAGEKNSHILDQRATREINAKMEQVLPRHSIPAFYISMNNLPQTATGKVDRRKLRIMGSKILSQKTHSTPSQQSQAAISSGTDTYTKLESIWITSLDLEPGSANMSATFFEMGGNSIIAIKMVNMARSNGIELKVSDIYQNPTLAGLKAIVIGTSLPYSLIPKVTRQGPVSEQSYAQNRMWFLDQLSEGASWYLIPFAVRMRGPVDVDALTRALLALEQRHETLRTTFENQDGVGVQIIHDRLSKELQVIDALDGDEGGLKTLYKVETTTFDITSEAGWSSTLIRLGKDDHILSIVMHHIISDGWSIDVLRRELIQLYAAALQGKDPSSALTPLPIQYSDFAVWQKQEAQAAEHERQLQYWKKQLADSSPAKIPTDFPRPDLLSGDAGVVPVAIDGELYQKLRGFCNKHNSTAFSILLAAFRAAHYRLTAVDDAVIGIPIANRNRWELENMIGFFVNTQCMRIAVDETDTFESLVRQVRSTTTAAFAHEDVPFERVVSALQPGHRDLSRTPLAQIMFAVHSQKDLGRFELEGIQSEPIASKAYTRFDVEFHLFQQADGLKGSCNFATDLFKPETIQNVVSVFFQILRHGLDQPETCISVLPLTDGVEELRRLDLLEIKRTNYPRDSSVVDVFREQAAANPEVIAVTDSSSRLTYAELDNKSELLSRWLRRRNLTPETLVSVLAPRSCETIVAYVGILKANLAYLPLDVRSPVTRMKDILSSVSGNTIVLMGSGVEDPGFDLPQLELVRITDTFDETIEDVQDSPQPSATSLAYVVFTSGSTGKPKGVMIEHRAIVRLVKSDNFPGFPSPARMSNVFNPAFDGAIWEINWMLLNGGTVVCIDYLTTLDGKELAAVFAKERVNAAFFAPAMLKLYLVDAREALKNLDFLIVGGERFDTKEAVEAMPLVRGKIANIYGPTEAGIISTCYNIPKDEAYTNGVPIGGSIYNSGAYVMDPNQQLVGLGVMGELVVTGDGVGRGYTNPELNKNRFIDITIEGKTFKAYRTGDRMRARVGDGLLEFFGRMDNQFKIRGNRIEAGEVESAMLSLKNVLNAAIVVRGGGEDEGPLEMVGFIVADDKNDTTEEEETGNQVEGWQDHFESGMYSDISTAVDQSAIGNDFKGWTSMYDGKDIDKGEMQEWLDDAIHTLHNGQIPRDVLEIGTGSGMILFNLNPGLNSYVGLDPSKSAVEFVNRAVESSPKFAGKAKVHVGMATDVNKLGEVHPDLVVFNSVVQYFPTPEYLAEVIDGLIAIPSVKRIFLGDIRSYATNGHFLAARAIHTLGTNNNATKDRVRQKIQELEDREEEFLVEPAFFTTLKERRPDVVKHVEIIPKNMKATNELSAYRYTAVVHLRDETDEPVYHIEKDSWVDFEAKQMDKTALLDHLRLSKDAMSVAVSNITYAHTAFERRIVESLDEDSKDDTKGTLDGAAWLSAVRSEAENRASLTVPDILEIAKEAGFRVEVSAARQWSQSGALDAVFHHFPPSSTDRTLIQFPTDNELRSSLTLANRPLQKLQRRRAALQVREKLQTLVPSYMVPPNIVVLDTMPLNTNGKIDRKELTRRARTLPKQQTAAPVPDFPISDIEITLCEEATEVFGMKVEISDHFFQLGGHSLLATKLISRIQHRLHVRVTVKDVFDSPVFADLAVIIRQGLAMQNPVAEGQDKQGWSSRVAPRTEVEKMLCEEFAAGLGVPVGITDNFFDLGGHSLMATKLAVRIGRRLIRHHSQGHLRLPCAFQLAKKLESSHSKSYEESGDDIQMADYTAFQLLDLEDPQDFVQSQIRPQLDSCYGTIQDVYPSTQMQKAFLFDPTTGEPRGLVPFYIDFPSNADAETLTKAIGALVDKLDMFRTVFLEAAGDLYQVVVEHLNLPIETIETEKNVNTATGDYLDVHGKDPVRLGHPCIQFAILKTASSVRVLLRMSHALYDGLSFEYIVRGLHVLYSGRNLPPPTQFARYMQYAAHSREEGYPFWREVLQNAPMTVLHDTNNGMSEQEMPASKAVHLSEVVNVPAQAIRNSTNTQATVFNTACALVLAKESGSQDVVFGRIVSGRQGLPVVWQDIIGPCTNAVPVHARVDDGNPQRIIRDLRDQYLRTLPFESLGFEEIKRNCTDWPEELTNFSVCVTYHNFEYHPESEVDNQKVEMGVLAKYVELSENEPLYDLAIAGEVEADGVNLKVTVVAKARLYNEARIRHVLEEVCKTFNGLNEAL