Organism
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
Function
May work in concert with and potentiate the processive hydrolytic action of muramidase-1, which requires binding of the enzyme to non-reducing ends of glycan chains. Hydrolysis in the midst of glycan chains would increase the number of binding sites for muramidase-1. May function in facilitating septum formation and cell separation. Active on M.luteus cell walls and on E.hirae cell wall fractions, but not active on E.hirae intact cell walls. Can covalently bind penicillin.
Similarity
Belongs to the glycosyl hydrolase 73 family.
Sequence
MENIARKERRRLNETKRFRKVKRSAALVGTAMVGCSVAAPLIQPVQVDADQTPTQFGARINTAAFIAEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAPYYNLFGIKGSYQGQTVYMDTLEYLNNKWVSKKEPFRQYPSFAESFNDNAYVLRNTSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIITTYGLTKYDTPASGNAGGGVTIGNGGNTGNTSNSGSTSGNSGGSATTTGTTYTVKSGDSVWGISHSFGITMAQLIEWNNIKNNFIYPGQKLTIKGGQSAGSSTTNTGNNASSGNTSGNTNTSGSTGQATGAKYTVKSGDSVWKIANDHGISMNQLIEWNNIKNNFVYPGQQLVVSKGSSSASGSTSNTSTGNTSSNTANTGSTTSGSTYTVKAGESVWSVSNKFGISMNQLIQWNNIKNNFIYPGQKLIVKGGSSSSNASTSTANNKNTASSNTSSTATGQATYTVKAGESVWGVANKNGISMNQLIEWNNIKNNFIYPGQKLIVKGGSSKASATATIKPTASTPASTTPTASSTGDTKYTVKAGESVWGVANKHHITMDQLIEWNNIKNNFIYPGQEVIVKKGTAQSTPAKSDEKTYTVKAGESVWGVADSHGITMNQLIEWNNIKNNFIYPGQQLIVKK