DBF4

DDK kinase regulatory subunit DBF4

704 amino acids

Regulatory subunit of the CDC7-DBF4 kinase, also called DBF4-dependent kinase (DDK), which is involved in cell cycle regulation of premitotic and premeiotic chromosome replication and in chromosome segregation. DDK plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. DBF4 recruits the catalytic subunit CDC7 to MCM2 and to origins of replication. DDK has also postreplicative functions in meiosis. DDK phosphorylates the meiosis-specific double-strand break protein MER2 for initiation of meiotic recombination. Interacts with CDC5 during meiosis to promote double-strand breaks and monopolar spindle orientation. Inhibits CDC5 activity during mitosis through direct binding to its PBD.

80.69 kDa

MVSPTKMIIRSPLKETDTNLKHNNGIAASTTAAGHLNVFSNDNNCNNNNTTESFPKKRSLERLELQQQQHLHEKKRARIERARSIEGAVQVSKGTGLKNVEPRVTPKELLEWQTNWKKIMKRDSRIYFDITDDVEMNTYNKSKMDKRRDLLKRGFLTLGAQITQFFDTTVTIVITRRSVENIYLLKDTDILSRAKKNYMKVWSYEKAARFLKNLDVDLDHLSKTKSASLAAPTLSNLLHNEKLYGPTDRDPRTKRDDIHYFKYPHVYLYDLWQTWAPIITLEWKPQELTNLDELPYPILKIGSFGRCPFIGDRNYDESSYKRVVKRYSRDKANKKYALQLRALFQYHADTLLNTSSVNDQTKNLIFIPHTCNDSTKSFKKWMQEKAKNFEKTELKKTDDSAVQDVRNEHADQTDEKNSILLNETETKEPPLKEEKENKQSIAEESNKYPQRKELAATPKLNHPVLATFARQETEEVPDDLCTLKTKSRQAFEIKASGAHQSNDVATSFGNGLGPTRASVMSKNMKSLSRLMVDRKLGVKQTNGNNKNYTATIATTAETSKENRHRLDFNALKKDEAPSKETGKDSAVHLETNRKPQNFPKVATKSVSADSKVHNDIKITTTESPTASKKSTSTNVTLHFNAQTAQTAQPVKKETVKNSGYCENCRVKYESLEQHIVSEKHLSFAENDLNFEAIDSLIENLRFQI

DBF4

Protein DBF4 homolog A

676 amino acids

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle (By similarity).

75.85 kDa

MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWEKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLVSNKKEAKYAQTLGQVSPVPSPESAYTAETTSPHPSHDGSSFKSPDRVCLSRGKLLAEKAVKDHDFIPANSILSNALTWGVKILHIDDIRYYIEQKKKELCSLKKSSTSVRDSGKKAGTTIQKARTGRLKKPFLKVEDVNRSYRPFYLQLTSVPSINYATHKPCSPFDIEKPSSVQKQAQPKPRPNTDGDKCGGTPVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHKNFAQSNQYQVVDDIVSKLVFDFVEYERDTPKKKRIKYSVGSFSSVSANVLKNTEPKEKLQLEPIFQKDMVESNGQLPEEIFQCEDIQCEDIQKPEQRLVLASEPMSYSSTGLKGRDEKAASMLNASEPDIKQKFTQLPPCKNEQEGILDVSEHKLIINRNDLEQRVGDSVGVPRSCVQVSHLSPENSLPQPKLTADTTHFSAKDLQEKDLHFVFGHDSDLVTLNTSKEQLTVKAGTPSCGPQQPNECDTENTDNLPCGKIQRKVRLLLGQKKKNVDPSAELDKKRTEFLPMCEDRTCGSPVQSLLDLFQTSGEKSDFLGFTSYTENSGLCDVLDVWEDENSSSLLSTFFSSPSASTFIGF

DBF4

Protein DBF4 homolog A

674 amino acids

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

76.858 kDa

MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Dbf4

Protein DBF4 homolog A

663 amino acids

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

74.176 kDa

MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKELGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGKLLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKTRTGRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLRINMDGDKCGTPVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHRNFAQSNQYQVVDDIVSQLVFDFVEYGRDTPQKKRIRYSVGSLSSVSANVLKNTAPKEKPLLEPNFQKDVGESSGHLLKPNSQYEETQKPEEKHGFASEPTTYSSAGLKGCDRKPVSMFNASEPDPEQEYAQLPLRDSTPEHQVTEGRNDGEQRVDPAPGVSQSCGQVSHLSTESNLPQPQLAADITQLSAKDLQEKGFHVVIGHASDLVALNTSKEQLTMKARTPPCSPQEPHECDTENMENLPCGKIQRKVRMLLGQQKANAEPSAELDKKRTEYLPAHEDRTCGSPVQSLLDLFQTSEEKSEFLGFTGYTENSGICDVLDIWEEENSSTLLSTFFSSPSTSAFVGF

dbf4

Protein DBF4 homolog A

663 amino acids

Regulatory subunit for cdc7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Not required during the initiation of DNA replication in egg and during early embryonic development but is required later throughout development. The complex cdc7-dbf4a phosphorylates mcm2 subunit (By similarity).

73.838 kDa

MKSTVATVKNPTGKVQADICKPFSGKVFYLDLTSKLISEKLEKDIKELGGTVEGFLSKEISYLITSKKEAKCVKTLKYVCSVPSPEPAQNTGESSTSPGNRRLPQEGNSSKKNEKSLVSRGKSLVKKAIKEQEILPKNSILSNALNWGVKILHVEEAKRYIEKKKSSLQQVKKSQPVVKSESKHPARRKVKPQKLKSPYIKVEDCSCQYRPLYLVLPQFRSFQNTTSNYLVEVDKKADVGQKLAETKQSINKTGHVQDGANNANIKLKEQKKHGYCECCLKKYDSLESHILSPQHKNYSESAYYQVVDDLISTFEFDFVDWSKYKNGRKSVGILMLTEKYKAEGQERNEASKANTFSERVSATTPLQENTLKDPYATSCSVPPTPVCNTDPMFSLPSPVGSAELCNKKYTTDNFEQLVATSVPALCLKDNLPGSLGEREMSVVYNETKQNETIDHTMERPKIGWDASNIPSNILLYVPQKVDAVAQYANVSLKGNIHCSKLTACQAALTYGKMDPAVCDNITFPKTVNHLHNKEGHRTMDQIYPTVQSDELQSLLPDYSPSGNLHRKLKTSAQTNLADDELLCRLSHKVSVPQQNDVLNVPSETLLAMFESSEDKTEFFGFAGSCVCDPCSMDDGDNRDQAHKNLLLSLFSHTTTSGSSFLGF

dbf4

Protein DBF4 homolog A

661 amino acids

Regulatory subunit for cdc7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Not required during the initiation of DNA replication in egg and during early embryonic development but is required later and throughout development. The complex cdc7-dbf4a phosphorylates mcm2 subunit.

73.699 kDa

MKSTVAAVKNPTGKVQADVCKPFTGKLFYLDLTSKLISEKLEKDIKELGGAIEGFLSKEISYLITSKKEAKCVKTLKYVCSIPSPEAAPNTGESSTSPGNRRLCQEGNSSKKNEKSLVSRGKSLVKRAIKEQEILPKNSILSNALNWGVKILHVEEAKHYIEKKKTALQQVRKSQPVVKVESKPQTRRKVKPQKLKSPYIKVEDCSCQYRPLYLVLPQFRSFQNPVSNYSVEVDKKADPGQKLPETKQSVNKTGHGQDGANNPNIKLKEQKKHGYCECCLKKYDDLESHILSPQHKNFSESAYYQVVDDLISTFDFDFVDWSKYKNGRKGVGILMLAEKSKTEGQERNEANLFVSKTHNFSERVTATTPLQENTLKDQHASPCSLPCTPACNADQMFSLPSPAGSARLCNKKYKMDSNFGQLVATPLPSFNLKDSLTGSFGGREMFVAKLNETMEPATEGPKIKWDVCNIPSNISQCIPQKVDPIAQHVNQFPNGNTHCSEMTACQAALIYENVDSAADANIKLPKTVNDLHNKEGHRATDKIYPTVQSHEYLPDYSPSGNLHRKVKTSAHRNKNKDELFCRLSHKVSVPQQEDDSKFPSETLLALFESSEDKTEFFGFAGSPAYESCSMDDGDTPDQTHKKMLLSLFPHTTTSGSSFLGF