Protein
D-galactosyl-beta-1->4-L-rhamnose phosphorylase
Organism
Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
Function
Reversibly phosphorolyzes beta-D-galactosyl-(1->4)-L-rhamnose to form alpha-D-galactose 1-phosphate and L-rhamnose. Does not phosphorolyze galacto-N-biose or lacto-N-biose. In the reverse reaction, has the highest activity toward L-rhamnose, also has activity toward L-mannose, and low activity toward L-lyxose, D-glucose, 2-deoxy-D-glucose and D-galactose.
Similarity
Belongs to the glycoside hydrolase 112 family.
Sequence
MEQQKEITKGGFTLPGEAGFEKLTLELANRWGADVIRDSDGTELSDDILNAGYGIYSTICLIRDHNAWAKANIDKLQQTFLVTSPVVANSETLTIDLMEGFFKEQFLVNDSEEALEYWQVYDRTTETLLQKESWSYHPQNQTVVLTGICPWHKYTVSFMAYRIWEEISMYNHTTNNWNKEHLMQIDPIHKETQEYLLTWMDDWCKKHEQTTVVRFTSMFYNFVWMWGSNEKNRYLFSDWASYDFTVSPHALKLFEEEYGYVLTAEDFIHQGKFHVTHMPADKHKLDWMEFINNFVVDFGKKLIDIVHNYGKLAYVFYDDSWVGVEPYHKNFEKFGFDGLIKCVFSGFEVRLCAGVKVNTHELRLHPYLFPVGLGGAPTFMEGGNPTLDAKNYWISVRRALLREPIDRIGLGGYLHLVEDFPDFTDYIEKIANEFRRIKELHNAGKPMALKPRIAVLHSWGSLRSWTLSGHFHETYMHDLIHINESLSGLPFDVKFINFEDINQGALEEVDVVINAGIMGSAWTGGQAWEDQEIIERLTRFVYEGKAFIGVNEPSALTGYDTLYRMAHVLGVDMDLGDRVSHGRYSFTEEPVEELEFAECGPKAKRNIYLTDGLAKVLKEENGIPVMTSYEFGRGRGIYLASYEHSIKNARTLLNIILYAAGESFHQEGITNNVYTECAYYEKDKILVMINNSNTLQESSVTIKGRTYTKDIPAFDTVILPLE