Protein
2-methylbutanal oxime monooxygenase
Organism
Manihot esculenta
Function
Catalyzes the conversion of (E)-2-methylpropanal oxime (valox) to 2-hydroxy-2-methylpropanenitrile (acetone cyanohydrin) and of (E)-2-methylbutanal oxime (ilox) to 2-hydroxy-2-methylbutyronitrile. The reaction takes place in three steps. First, the oxime is isomerized to the (Z)- isomer, next the (Z)-isomer is dehydrated to the corresponding nitrile, followed by a C-hydroxylation of the nitrile. Can use both aliphatic and aromatic oximes as substrates.
Similarity
Belongs to the cytochrome P450 family.
Sequence
MSVAILTSLPPQWLSILAVFLLPILTLLLFRGKDDNQKKGLKLPPGPRQLPLIGNLHQLGGQPYVDFWKMAKKYGPVMYLQLGRCPTVVLSSTETSKELMKDRDVECCSRPLSVGPGQLSYNFLDVAFSPYSDYWREMRKLFIFELLSMRRVQTFWYAREEQMDKMIEILDGAYPNPVNLTEKVFNMMDGIIGTIAFGRTTYAQQEFRDGFVKVLAATMDMLDNFHAENFFPVVGRFIDSLTGALAKRQRTFTDVDRYFEKVIEQHLDPNRPKPETEDIVDVLIGLMKDESTSFKITKDHVKAILMNVFVGGIDTSAVTITWAFSELLKNPKLMKKAQEEVRRAVGPNKRRVEGKEVEKIKYIDCIVKETFRKHPPVPLLVPHFSMKHCKIGGYDILPGTTIYVNAWAMGKDPTIWENPEEYNPDRFMNSEVDFRGSDFELVPFGAGRRICPGLAMGTTAVKYILSNLLYGWDYEMPRGKKFEDFPLIEEGGLTVHNKQDIMVIPKKHKWD