Function
Chlorophenol O-methyltransferase that methylates chlorophenols into chloroanisoles which are thought to be responsible for cork taint of wines (PubMed:20144725, PubMed:12957890). The only single chlorophenol (CP) methylated is 2-CP; neither 3-CP nor 4-CP are effective substrates (PubMed:12957890). Within the dichlorophenols (DCPs), 2,4-DCP supports the highest rate of O-methylation, and the activity decreases in the following order: 2,3-DCP, 2,5-DCP, 2,6-DCP, and 3,4-DCP (PubMed:12957890). Within the trichlorophenol (TCP) group, the maximal activity is observed with 2,3,4-TCP, whereas there is increasingly reduced activity with 2,4,5-TCP, 2,4,6-TCP, and 2,3,6-TCP (PubMed:12957890). The only tetrachlorophenol (TeCP) that is methylated is 2,3,4,5-TeCP, since no activity can be detected with 2,3,4,6-TeCP and 2,3,5,6-TeCP (PubMed:12957890). Is also able to methylate other halogenated phenols containing fluoro or bromo substituents, whereas other hydroxylated compounds, such as hydroxylated benzoic acids, hydroxybenzaldehydes, phenol, 2-metoxyphenol, and dihydroxybenzene, were not methylated (PubMed:12957890).
Sequence
MAELRAPSSLSTERNGSASNTDVDKQKLNHLYQNGNKKTGSAESRMLVLAETIRAETQKLHAYLESNGIAQPDLSVDAPDDFPPLPDEIQESRQKIFLASRELTDIVRGPRETVRYAVWSYLDTLSLQLINSYGIAKLVPLDAPIKLTELQSKTPLEPVHLARALRHAMTNNIFREPSPGYIAHTSSSRILAQDPALQAWVGFNSEDAFPAAGHVLQALKDHPEAISSTHAGFNYAFNTVGQEPMFATLGKDLARAKRFAQAMHSFSHGEGYKVSYFVDNYDLSEVDKRGGTFVDIGGSHGFVSVELAKRWKDMKFIVEDLPKTIESAPQPISDDKTVADRISLQAHDFFQEQPVKGADVYFFRWIIHNHAKPYAVSILRNLIPALKPGARVVINDYCIREAGSENAWDEKLLRNMDMIMGALLNAQEREEWEFRELFEAADPRFKFKGVQRVENCKMSVIEAVWDE