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C10

Gene
C10
Protein
Para-Rep C10
Organism
Milk vetch dwarf virus (isolate N)
Length
283 amino acids
Function
Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Similarity
Belongs to the nanoviridea/circoviridae replication-associated protein family.
Mass
33.383 kDa
Sequence
MPSIRAIHWCFTLNFSGKIPEIVWTADVQYSIWQHERVNHDHLQGYIQMKKQTTLKKMKELLPGAHLEMARAPKKAIDYCQKKETAIDGPWEYGTWISTGSHKRKLMERFDEDPEEMKLEDPGLYRRCLSRVQMTKVREKNSWDYDLRPWQDELLKTIEQEPDDRTILWVYGPHGGEGKSVFAKYLTLKEGWWYTAGGKATDMLYSYSLDPTCHVCIDIPRCTKEEYINYAVIEQIKNRIIINTKYEPCTIRDDGHNVHVIVFANYLPDVTRISEDRIKIIYC