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Bphyt_4144

Gene
Bphyt_4144
Protein
Nitronate monooxygenase
Organism
Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
Length
366 amino acids
Function
Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. Acts on propionate 3-nitronate (P3N), the presumed physiological substrate (PubMed:25002579). Is likely involved in the degradation of P3N, that allows B.phytofirmans PsJN to grow on 3-nitropropionate/P3N as the sole source of nitrogen and carbon (PubMed:20382807). Also probably functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism (PubMed:25002579). Cannot oxidize nitroalkanes such as 3-nitropropionate, nitroethane, or 1-nitropropane (PubMed:25002579).
Similarity
Belongs to the nitronate monooxygenase family. NMO class I subfamily.
Mass
38.062 kDa
Sequence
MTDQTGKRALLPLLGIDKPIIQAPMAGVSTPALAAAVCNAGGLGSLGVGAMNADGARKVIRETRALTDKPFNINVFCHRPAQADAAVEQQWLSWLAPHFEKYGATPPAKLSDIYTSFLADPAMLAVFLEEKPAIVSFHFGLPSADVIAELKKAGIRLLASATNLQEAAQVEAAGVDAIVAQGIEAGGHRGVFDPDAFDDRLGTFALTRLLAKECRLPVIAAGGIMDGAGIAAALALGAQAAQLGTAFVACTETSIDEGYRRALLGEAARRTTFTAAISGRLARSMANSFTALGADPRSPEPATYPIAYDAGKALNAAAKAKGEFGYGAHWAGQAAALARSLPAAELVAQLERELKQSIEQLRQFAN