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AO090010000708

Gene
AO090010000708
Protein
NADPH-dependent reductive aminase
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40)
Length
295 amino acids
Function
NADPH-dependent reductive aminase that catalyzes the reductive coupling of a broad set of carbonyl compounds with a variety of primary and secondary amines (Ref.2). Possesses remarkably high activity for the reductive amination of ketones and amines, often with high stereoselectivity and in some cases with ketone:amine ratios as low as 1:1 (Ref.2). The cofactor NADPH, the carbonyl compound and the amine are added to the enzyme in that sequence, followed by the release of product, NADP(+) being released at last (Ref.2). RedAm is also able to act in the reverse, oxidative direction and exhibits activity in the dehydrogenation of amines to yield imines (Ref.2). The highest activity is found for 1-methyl-tetrahydroquinoline and acyclic amines are also found to be transformed (Ref.2).
Similarity
Belongs to the HIBADH-related family.
Mass
31.155 kDa
Sequence
MSKHIGIFGLGAMGTALAAKYLEHGYKTSVWNRTTAKAIPLVEQGAKLASTISEGVNANDLIIICLLNNQVVEDALRDALQTLPSKTIVNLTNGTPNQARKLADFVTSHGARYIHGGIMAVPTMIGSPHAVLLYSGESLELFQSIESHLSLLGMSKYLGTDAGSASLHDLALLSGMYGLFSGFLHAVALIKSGQDTSTTATGLLPLLTPWLSAMTGYLSSIAKQIDDGDYATQGSNLGMQLAGVENIIRAGEEQRVSSQMILPIKALIEQAVGEGHGGEDLSALIEYFKVGKNVD