Protein
Arachidonate 12-lipoxygenase, 12R-type
Organism
Rattus norvegicus
Function
Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.
Similarity
Belongs to the lipoxygenase family.
Sequence
MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLPKDPWYCNYVQICAPNCRVYHFPAYQWMDGYETLSLREATGKTTADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWNGYIPGFPILINIKATRFLNLNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKTVVSEYVAEHWTEDSFFGYQYLNGINPGHIRRCMQIPDKFPVTDEMVAPFLGEGTCLQAELEKGNIYLADYRILDGIPTVELNGQKQHHCAPICLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLERESSGFPTCLRTVPELIEYVTMVMYTCSARHAAVNTGQLEYTSWMPNFPSSMRNPPMQSKGLTTLQTFMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEAPRRSMEAFRQNLNQISHNIRQRNKCLNLPYYYLDPVLIENSISI