Protein
Cytosolic carboxypeptidase 3
Function
Metallocarboxypeptidase that mediates both deglutamylation and deaspartylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates or polyaspartates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.
Similarity
Belongs to the peptidase M14 family.
Sequence
MSEDSEEEDYSDRSISDDDDLDEDSFMKFVSDDIHPCTLLAADSIGDPFFPRTTQILLEYQLGRWVPRLRGPRDLYGVSSSGPLSPTRWPYHCEVIDEKVQHIEWTPFVPEPVYVPTGLEIEPVYPNSKEDTVVYLAEDDHLCKAYKEPCFVYSRVGGNRTSLKQPVDNCDNTLVFEARFESGNLQKVVKVADHEYELTVRPDLFTNKHTQWYYFQVTNTQAEIVYRFTIVNFTKPASLYNRGMKPLFYSEKEAKTHNIGWQRIGDQIKYYKNNLGQDGRHFFSLTWTFQFPHSQDTCYFAHCYPYTYSNLQEYLSGINSDPVRSKFCKIRVLCHTLARNMVYVLTITTPLKTSDSKRKAVILTARVHPGETNSSWIMKGFLDYILGDSSDARLLRDTFIFKVVPMLNPDGVIVGNYRCSLAGRDLNRNYTSLLKESFPSVWYTRNMINRLMEKREVILYCDLHGHSRKQNIFMYGCDGSSRSKTKGLYLQQRIFPLMLSKNCPNIFSFSACKFNVQKSKEGTGRVVMWKMGIRNSFTLEATFCGSTLGNKRGTHFGTKDLESMGYHFCDSLLDYCDPDRSKYYQCLKELEEMEKHLSSERVSDNTDTSLVEISLDVESSSRGSDSSESNDTQTYLLKVTSQARNKKKYLKTKRERNAILANCQNNMQEVYGKEHLLQRHDESNSDGNDPRIDAPDVYVAHCFRRPLPNQGVVKIPGQRFYPGKTWSSSQRMIKSLNKDHRTCILETCKNPIQEVQSRGINIHESCFKMAKCPMNKRPSHWIEKTRIPTESHHQLKSKAKRCSSFQSKRTGTNWTDDEKRIYRDKRIAQTQEILKYLLPIVESSQNRKSTQMNNLINPIANLQQHQLIPTACINRRRYSIPWTPTRNLPFKAQRNLMTDTSEWLQSVPLGSFESLLPLCNLQKKTKHFELWGKKAKDVQLATSQWEAVPLSSNMDASIIRGNSVLQPKEFTMRSSKQRIPYLTKTSKKPSESDGLLTFQLKIHRNS