Protein
Cytoplasmic tRNA 2-thiolation protein 1
Organism
Anopheles gambiae
Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Similarity
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Sequence
MPVPCGSSCGRNAFMRRPKTGDTLCKECFFLAFETEIHNTIQQEQLFRPGEKVAIAASGGKDSTVLAHVMNLLNKRYNYGLDLVLLSIDEGITGYRDDSLKTVAQNRDDYGMPLRVLSYQELYGWTMDRIVAEIGRSNNCTFCGVFRRQALDRGARLMEVDCVATGHNADDIAETVIMNILRGDTARLRRCCDIKTGSKEADTIPRVKPLKYSYEKEIVMYAHFKKLVYFSTECVFAPNAYRGHARAFLKDLEKVRPSAIMDIIHAGEQLQIKGTVKKPVRGVCGRCGFVSSQQPCKACVLLEGLNRGLPKLGIGKKSKGERMVALQEQQLREKAHLVKNDF