Protein Design Games (Winter 2024)

A collaboration between Liberum Biotech and Rosetta Commons

Results

Activity Assay:

Contestant DNA was resuspended in water and 10 ng was used as template in a 25 µL PCR reaction with Q5 DNA polymerase. DNA was amplified using a standard cycling protocol and 3.3 µL was used as template for 33.3 µL cell-free reactions. Each reaction was diluted 100X and the TEV protease activity assay was carried out using the SensoLyte® 520 TEV Protease Assay Kit. Fluorescence at 520 nm was recorded at the 20 min time point.

Scores:

Liberum is proud to announce the winner of this the Protein Design Games (Winter RosettaCon 2024) is Team 6. The team designed the three top-performing TEV protease sequences and will take home the cup, the bragging rights and the monetary award generously offered by the RosettaCommons.

The winning participants who submitted their designs for Team 6 are as follows:

Russell Ault (Children's Hospital of Philadelphia)

Sam Garfinkle (University of Pennsylvania)

Andrew Nelson (University of Pennsylvania)

Colby Agostino (The Wistar Institute)

Shahlo Solieva (The Wistar Institute)

Michaela Helble (University of Pennsylvania)

Caroline Davis    (University of Colorado)

Team #	Communication Score	Activity Assay Score	Total
1	3	4.5	7.5
2	3	1.1	4.1
4	3	5.7	8.7
5	3	4.3	7.3
6	3	7.0	10.0

Important Note:

None of the Winter RosettaCon organizers were involved in running the experiments or judging the results.