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PHYPADRAFT_141055 recombinant protein | tRNA (guanine(37)-N1)-methyltransferase

Description
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Family
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Species
Physcomitrella patens subsp. patens
Length
500 amino acids
Sequence
MHHHHHHSGGGGSENLYFQSMAAPGCSPVGIDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAIAQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETIGHIAHLNLREDLLTYKKIIAEVILDKNPKLKTVVNKVGTITNEFRVPEFEILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKLVTEEEGCVGEIASSTLIAENSLNGTRIPSSKENSSSLTEGSDMPSSKKPEERKAKKAKVSEVVKMKISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKASLAFTAVVSWFISRSFIWSLYFFCLIQAHLVLDSIEHR
Mass
55.9 kDa
SDS-PAGE
Western blot of PHYPADRAFT_141055 recombinant protein(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Purity
Greater than 85% as determined by SDS-PAGE (lot specific).
Expression
E. Coli Cell-Free System
Format
Lyophilized or Frozen (format to be determined during manufacturing)
Tags
N-terminal 6XHis-tag, TEV protease site and linker.
Sterility
Sterile filtering available upon request.
Endotoxin
Low endotoxin available upon request.
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.