Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Physcomitrella patens subsp. patens
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Greater than 85% as determined by SDS-PAGE (lot specific).
E. Coli Cell-Free System
Lyophilized or Frozen (format to be determined during manufacturing)
N-terminal 6XHis-tag, TEV protease site and linker.
Sterile filtering available upon request.
Low endotoxin available upon request.
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.