Protein expression services for ileS | Isoleucine--tRNA ligase
Description
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
Family
Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.
Species
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Length
1054 amino acids
Sequence
MTEAVGGVYPLVDMTGGSSRFPEMEENVLDFWKRDDTFQASIDQRNDAEDYVFYDGPPFANGLPHYGHLLTGYVKDIVPRYQTMRGYRVPRVFGWDTHGLPAELEAEKQLGIKDKGEIESMGLAQFNDYCAKSVLQYTKEWEEYVTRQARWVDFENGYKTMDLTYMESVIWAFKELYDKGLIYQGFRVLPYSWAEHTPLSNQETRLDDSYKMRQDPTLTVTMPITGKIEGTGANEALIGANAIAWTTTPWTLPSNLALAVNPSVTYALVEVAGDGEEGFVGQQLLLARDLVGAYAKELGTDARIISEHPGTELIGLTYEPVFDFFRDHPNAFQILGADYVTTEDGTGIVHQAPAFGEDDMNVCNAADIAPVIPVDMDGKFTSLTPEYEGQLVFDANKDIIRDLKAKGRVFRHQTIEHSYPHSWRSGEPLIYMALPSWFVNVTKIRDRMVETNQDIEWMPAHIRDGQFGKWLEGARDWNISRSRYWGSPIPVWVSDDENYPRVDVYGSLDELEADFGVRPTSLHRPYIDELTRPNPDDPTGKSTMRRVPDVLDVWFDSGSMPFAQVHYPFENKDWFDTHAPADFIVEYIGQTRGWFYLLHVLSVGLFDRPAFKKVVAHGIVLGDDGLKMSKSKGNYPNVTEVFDRDGSDAMRWFLMSSPILRGGNLIVTEKGIREGVRQAQLPMWNAYSFLQLYASKKATWSVDSTDVLDRYILAKLHDLVADVTAALDATDIARACDQVRWFCDALTNWYVRRSRDRFWAGDEAHPEAFNTLYTVLETLTRVAAPLLPMTTEVIWRGLTGERSVHLTDFPSADSFPADADLVRTMDEIRGVCSAASSIRKAHKLRNRLPLPNLTVALPDSGRLADFLSIIRDEVNVKNVDLTSDVDAVGTFEVVVNAKVAGPRLGKDVQRVIKAVKAGNYERVGETVVADGIELQDGEYTERLVAANPDSTAQIDDVDGLVVLDMEVTPELEAEGWAADVIRGLQDARKSSGFEVSDRIQVTLSVPGDKQEWATRHADHIAGEVLATSFEVTQDDLGEDAHEVLKGVTASVARV
Mass
118.2 kDa
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service
Make ileS using our protein expression services starting at $99 + $.30/amino acid in as fast as two weeks (includes the cost of DNA synthesis)
Order Here