Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

915 amino acids

MAKPETENPYSKTVLLPETSFPMKADLAKREPGQIKVWKDQKVFLNMKEIRKSKPSFVLHDGPPYANGNFHVGHSLNKILKDIIIKSKTLSGYQTDMIPGWDCHGLPIEVQVLKNLGKEARNTGPSELRKKCREYAAEFVGKQGEDLNRFLCFWDENNKYLTMAPEFEARIVEVFGSLFAKGYIYKGKKPVYWCIDLATAHAEAEIEYQNHVSPSIYVKFAVKGETDTHCLIWTTTPWTLPANLAICFNEELPYSLFQSDAHGRLILADGLKEAVEQKTGITLTKIKSLSNADLKQMVFLHPFLDRESIPLFGNHVTLDAGTGCVHTAPGHGTDDYRVGTAAGLPTLSPVDDYGRYTDEFEMMKGIKIWDANPKIVELLREKNALVHFSEFTHSYPHSWRSKKPLIFRATPQWFFSIDHNGLRDESLKAIDKVQWIPDWGITRIRSMVESRPDWCLSRQRNWGVPIPSFTCKSCGLTHLDDKTIQHFIQIVKKEGIEVWYEKEAKDLLPADTKCSNCGSEDLKQDKDILDVWFDSGVSSFAVFGDSIGKEPADLYLEGSDQHRGWFQSSLWPSMAIRKTPPYKSVLTHGYVLDEKGHAMSKSLGNVINPTTDIINQYGADILRLWVSTQDFRDDVKIGKDSIKTVSEAYRKIRNTFRYLLGNTSAETLTWNLKKEELDTIDKYYLHKLAKLNDEVKKLYDTYQFHQVYHKILGFCTVDLSQDYFEIIRDRMYCDAKESKTRRSSEYTLAVILEVLSKLLAPILSFTTEEVWTSFGKKDSVFYSDFSDLTEWLDESLESKMKPVFETKEDVQKALEEARKLGKLGKSLEAEVVIDGKKDSLPFSSEELALFFVVSHVHFETNGIQEVFSEWKGESGSIQIRKPKHFECPRCWRHVSETEGKLCKRCDEVVSKLSPN

104.7 kDa

(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)

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