Description
Catalyzes the removal of dipeptides from the N-terminus of oligopeptides. Most potently cleaves the synthetic substrate Met-Leu-methylcoumaryl-7-amide (Met-Leu-MCA), followed by Leu-Arg-, and to a much lesser extent Lys-Ala-, Leu-Asp-, Leu-Glu-, Leu-Lys, and Val-Arg-MCA, while this enzyme does not hydrolyze Gly-Arg-, Gly-Gly-, Lys-Lys-, or Gly-Pro-MCA.
Family
Belongs to the peptidase S46 family.
Species
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Sequence
MNRLKLYLLALTALAVCSAKADEGMWLLQLMQQQHSIDMMKKQGLKLEAQDLYNPNGVSLKDAVGIFGGGCTGEIISPEGLILTNHHCGYASIQQHSSVEHDYLTDGFWATSRDKELPTPGLKFTFIERIEDITDIVNLRIAAKEITESESFSSTFLNKLAKELFEKSDLKGKKGIVPQALPFYAGNKFYMFYKKVYPDVRMVAAPPSSIGKFGGETDNWMWPRHTGDFSMFRIYADANGEPAEYSASNVPLKTKKHLNISIKGLKEGDYAMIMGFPGSTSRYLTVSEVKERMEASNAPRIRIRGTRQDVLKEAMNASDKVRIQYANKYAGSSNYWKNSIGMNKAIIDNNVLGTKAEQEAKFAKFAKEKNNTDYMNVVAKIDEAVAKTSPIKYQQTCLTETFFGGIEFGSPFMVMDKLKEALEQKNDSSIEANIKVLKEVFNDIHNKDYDHEVDRKVAKALLPLYAEMIPAGQRPAIYDVIEKEYKGDYNAYVDAMYDTSILANQANFDKFIKKPTVKAIEKDIATQYSRAKFDKYTNLAEQMGKLPEELALLHKTYIRGLGEMKLPVPSYPDANFTIRLTYGNVKPYSPKDGVYYKYYTTTDGILEKENPEDREFVVPAKLKELIEKKDFGRYALPNGEMPVCFLSTNDITGGNSGSPVLNENGELIGCAFDGNWESLSGDINFDNNLQRCINLDIRYVLFILEKLGGCGHLINEMTIVE
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service