Description
Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation. Can transfer a number of fatty acids (C16 and C19, palmitic and probably tuberculostearic acids respectively are shown) (PubMed:19661058). Enhances the polyprenol monophosphomannose (PPM) synthase activity of Ppm1 (AC A0QZ12) without itself having PPM synthase catalytic activity (PubMed:12427759).
Family
Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.
Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Sequence
MIPAVTDDDPLEDPLDDDVAPGLDDAEPEPEPRDEHDEPSRPATGSRIGGWVARRGSRFGKGVLDRCAPLSAAIGGGLALWLSFPPIGWWFTAFPGLALLGWVLTRTATTKAGGFGYGVLFGLAFYVPLLPWISGLVGAVPWLALAFAESLFCGLFGLGAVVVVRLPGWPLWFATLWVAAEWAKSTFPFGGFPWGASSYGQTNGPLLALARIGGAPLVSFAVALIGFSLTLLTAQIVWWWRHGHKPGVPAPAVMLPGVAIAASLLVTALVWPQVRQSGTGAGDDTAVTVAAVQGNVPRLGLEFNAQRRAVLDNHVKETLRLADDVKAGRAAQPMFVIWPENSSDIDPLLNADASAQITTAAEAIDAPILVGGVVRADGYTPDNPVANNTVIVWEPTDGPGERHDKQIVQPFGEYLPWRGFFKHLSSYADRAGYFVPGTGTGVVHAAGVPIGITTCWEVIFDRAARESVLNGAQVLAVPSNNATFDEAMSAQQLAFGKLRAVEHDRYVVVAGTTGISAVIAPDGHEISRTEWFQPAYLDNQIRLKTDLTPATKWGPIVQAVLVIAGVAVLLIAILHNGRFAPRMLRRRSATTVKR
Simulated SDS-PAGE
(Note: Representative image - actual molecular weight may vary depending on tag type and expression method)
Safety
Upon ordering, we will perform rigorous biosecurity and export control screening to ensure that order fulfillment is consistent with all legal and regulatory guidance.
Protein synthesis service