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vlmH

Gene
vlmH
Protein
Isobutylamine N-hydroxylase
Organism
Streptomyces viridifaciens
Length
378 amino acids
Function
Involved in the biosynthesis of the azoxy antibiotic valanimycin, which has an antitumor activity (PubMed:9056232, PubMed:8990292, PubMed:9287340). Catalyzes the oxidation of isobutylamine to isobutylhydroxylamine via the formation of a flavin 4a-hydroperoxide (PubMed:9056232, PubMed:8990292, PubMed:9287340). Unlike other known N-hydroxylases, isobutylamine N-hydroxylase cannot carry out the reduction of the flavin cofactor and requires the NADPH-flavin oxidoreductase VlmR (PubMed:9056232). Also able to oxidize propan-1-amine, butan-1-amine, butan-2-amine and benzylamine (PubMed:9056232). It has a similar activity with either FMNH(2) or FADH(2) (PubMed:9056232).
Mass
39.89 kDa
Sequence
MRSLDAARDTCERLHPGLIKALEELPLLEREAEGSPVLDIFRAHGGAGLLVPSAYGGHGADALDAVRVTRALGACSPSLAAAATMHNFTAAMLFALTDRVIPPTDEQKKLLARVAPEGMLLASGWAEGRTQQDILNPSVKATPVDDGFILNGSKKPCSLSRSMDILTASVILPDETGQQSLAVPLIMADSPGISVHPFWESPVLAGSQSNEVRLKDVHVPEKLIIRGTPDDPGRLDDLQTATFVWFELLITSAYVGAASALTELVMERDRGSVTDRAALGIQLESAVGLTEGVARAVRDGVFGEEAVAAALTARFAVQKTLAAISDQAIELLGGIAFIKSPELAYLSSALHPLAFHPPGRTSSSPHLVEYFSGGPLEI