Protein
Validamycin A dioxygenase
Organism
Streptomyces hygroscopicus subsp. limoneus
Function
Involved in the biosynthesis of validamycin B, a component of the antifungal and antibiotic validamycin complex used as a crop protectant. Catalyzes the regioselective hydroxylation of validamycin A (4-O-beta-D-glucopyranosyl-validoxylamine A) at the C-6 position to yield validamycin B. To a lesser extent, also able to convert validoxylamine A to its hydroxylated derivative.
Similarity
Belongs to the iron/ascorbate-dependent oxidoreductase family.
Sequence
MTGSVPIVDLEAWRAADEENRASLAEIIDGALHTVGTFLLAGHGVPAELTARMRTAGRSFFDLPWEKKEPHAVQRPHDNGWRGLVKHRTDTIEGTGGAPDLHEAFHMGPTHRTGDDAFDALYYPANKWPAELPELRETALAYTAHMTRVAGAVMEMLAGVLGLEPAFFTSRCEHATWTQSVNWYPSLDTVGQTAEGQMRVGPHTDFGTITLLDRQQGVSGLEVWSEEDGWFAPPFVEGTLLVNLGDLMHQWTDGRWRSLRHRVLAPSASAPQEELVSLVYFFDADPEAELVPLAAPVGGGAGMPTVNVGETILKKNIQMLTDLKGHGLFQGELSLSRPGSADSPGSSPADDHPSRPGRHPAQGPQ