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vha-1

Gene
vha-1
Protein
V-type proton ATPase 16 kDa proteolipid subunit 1
Organism
Caenorhabditis elegans
Length
169 amino acids
Function
Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Required along with other vacuolar ATPase components for the removal of protein aggregates which form in immature oocytes in the distal gonad (PubMed:29168500). This removal occurs as the oocytes mature and move to the proximal gonad, is triggered by the introduction of sperm through mating and occurs before fertilization (PubMed:29168500). The introduction of sperm triggers V-ATPase accumulation in proximal oocytes and induces lysosomal acidification which leads to engulfing of protein aggregates by lysosomes and subsequent clearance of the aggregates (PubMed:29168500). Lysosomal acidification also leads to changes in mitochondrial morphology and function (PubMed:29168500). Mitochondria in distal immature oocytes are fragmented, produce high levels of reactive oxygen species (ROS) and have high membrane potential, indicative of metabolic inactivity (PubMed:29168500). In contrast, mitochondria in proximal mature oocytes are tubular with lower ROS levels and membrane potential, indicative of an active metabolic state required for aggregate mobilization before clearance (PubMed:29168500).
Similarity
Belongs to the V-ATPase proteolipid subunit family.
Mass
16.954 kDa
Sequence
MSTDTKQIIADALLKNEQAMYGPFFGSLGVTSAMAFAAAGSAYGTAKAGTGIASMAVARPDLVMKAIIPVVMAGIVAIYGLVVAVIVSGKVEPAGANYTINNAFSQFAGGLVCGLCGLGAGYAIGIAGDAGVRALSQQPRMFVGMILILIFAEVLGLYGMIVALILGAT