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ttuA

Gene
ttuA
Protein
tRNA-5-methyluridine(54) 2-sulfurtransferase
Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Length
321 amino acids
Function
Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (PubMed:16547008, PubMed:28439027). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures (PubMed:16547008). TtuA transfers the S atom from the thiocarboxylated C-terminus of TtuB to tRNA (PubMed:28439027).
Similarity
Belongs to the TtcA family. TtuA subfamily.
Mass
36.194 kDa
Sequence
MVCKVCGQKAQVEMRSRGLALCREHYLDWFVKETERAIRRHRMLLPGERVLVAVSGGKDSLALWDVLSRLGYQAVGLHIELGIGEYSKRSLEVTQAFARERGLELLVVDLKEAYGFGVPELARLSGRVACSACGLSKRYIINQVAVEEGFRVVATGHNLDDEAAVLFGNLLNPQEETLSRQGPVLPEKPGLAARVKPFYRFSEREVLSYTLLRGIRYLHEECPNAKGAKSLLYKEALNLVERSMPGAKLRFLDGFLEKIRPRLDVGEEVALRECERCGYPTTGAVCAFCRMWDAVYRRAKKRKLLPEEVSFRPRVKPLRAG

Gene
ttuA
Protein
tRNA-5-methyluridine(54) 2-sulfurtransferase
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Length
310 amino acids
Function
Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (PubMed:28655838). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures (By similarity). Can use free sulfide as sulfur source in vitro, which may be also the sulfur source in vivo (PubMed:28655838).
Similarity
Belongs to the TtcA family. TtuA subfamily.
Mass
35.633 kDa
Sequence
MKCKFCSREAYIKIHYPKMYLCEEHFKEYFERKVSRTIERYKLLTKDERILVAVSGGKDSAVTAYVLKKLGYNIECLHINLGISGYSEKSEEYAKKQCKLIGAPLHIVRIKEILGYGIGEVKTRRPPCSYCGLTKRYIMNKFAYDNGFDAIATGHNLDDEASFLLNNILHWNTEYLAKGGPILPQQGKFIKKVKPLYEVTEREVVAYALAVGLEYIVEECPYARGATTLDMKGVLNELEEKRPGTKFNFVRGYLKKKKLFEPEIKEKEIKECKICRMPSSGDICAFCKFWGLKKEINFKVSSTDEEPFGP

Gene
ttuA
Protein
HTH-type transcriptional regulator TtuA
Organism
Agrobacterium vitis
Length
304 amino acids
Function
Transcriptional regulator of the ttuABCDE tartrate utilization operon.
Similarity
Belongs to the LysR transcriptional regulatory family.
Mass
33.903 kDa
Sequence
MELNQLRCFVSVADELHFGRAAQKLGLMPASLSRHIRMLEESLGTRLMARTTRSVALTDDGLFLLEGAREILERTEVLQNRFQTSRQERVEILRIGAIDSAAAGLLPTLLHDFRQQCPEISTQLSEDKTIRLIPKLLSGGLDLAFIRAPDFVDPKLKTVFLFHETAVVAMSENHRLAHNTSVTIHDLADEPLIVPERRSRPHSHDLTMKLFIEAGLQARVAQLAEEKQTIVNLVAAEIGLAIVPQWTARLAVSGVRYVTLQTDAKEAMNKLPLSAAWLKGVRDPARDLIVSLLQSRISQYAENA

Gene
ttuA
Protein
HTH-type transcriptional regulator TtuA
Organism
Agrobacterium vitis
Length
304 amino acids
Function
Transcriptional regulator of the ttuABCDE tartrate utilization operon.
Similarity
Belongs to the LysR transcriptional regulatory family.
Mass
33.854 kDa
Sequence
MELEQLKCFVAAAEELHFGRAAQKMGILPASLGRHLRLLEESLGTRLMSRTTRSVALTEDGVALLEEVRPLLHRLQTLAEEFRSRKTQQATVLRIGAIDSAAAGLIPPLLHDFRERFPGIITQLVEDKSIRLIPKLIAGRLDIVFIRPRDGLNRNLVVRTLTQETPVIALPVTHPLANRERISVDELREEPLIVPERRSRPHSYDLTMKLFEDAGLHPRIAQTADEKQTIINLVAAGIGSAIVPRWTAKLAVFGVTFVPLMTVGGISLRKLPLAAAWAKAVRDPGRDQLLELLDTNIDRYAEQA

Gene
ttuA
Protein
tRNA-5-methyluridine(54) 2-sulfurtransferase
Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Length
304 amino acids
Function
Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (PubMed:28655838). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures (By similarity). Can use free sulfide as sulfur source in vitro (PubMed:28655838).
Similarity
Belongs to the TtcA family. TtuA subfamily.
Mass
34.934 kDa
Sequence
MKCTKCGKPASVKLRHYNIKLCKEHFNEFIEQRVEKAIKKFKMFGRNSKILIAVSGGKDSVSLWHMLKKLGYEVDALFIRAGKSGMVQKAQEIVEKNAELLNTKLHIVDATEYFGGLSTQEISIMLRRPVCSICGVVRRYLMNKFAYENGYDVVVTGHNLNDEASVLLGNILHWQEGYLERQWPLLPKTHEKLVPKAKPLVLNYEEDIKLYATLNEIPHLEMACPFSVGATSLVYKKILRELEEEQPGITLNFYLGFLKRKKEPKFEVEGLRECKECGYPTTAEVCSFCRLRKQVEKRKNKTPA