Protein
Type-2 restriction enzyme TthHB8I
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Function
Recognizes the double-stranded sequence TCGA and cleaves after T-1.
Sequence
MAPTQAQKVLEAFEDFLKCLDLESYQEKYRPIKTVEQDLPRELNPLPDLYDHYWKPNGNTLHFPDFETFFDQWWEKRLRPLNEFIRKYFWGCSYEFVRLGLEARLYRTAVSIWTQFHFCYRWNASCQLRLTATWELDAQGIDAQIQAEDRLIGIQIKKETYRSEAREGNRFLRRREHSALLEVPYTLQSPEELERKAQRARTREEAYRLWVKIAHHLERLPNGFVIFRESYVKDLENFLKQNATTLSGLIPWDKVAREALTGP