Protein
Thymidylate synthase 2
Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Similarity
Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence
MKQYKDLCRHVLEHGEKKGDRTGTGTISTFGYQMRFHLQEGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIKTNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLSCQLYQRSADVFLGVPFNIASYALLTMMIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLTRDVRPLPKLRFAREIDSIFNFAFEDFIIEDYDPHPHIKGAVSV