Protein
RNA 2',3'-cyclic phosphodiesterase
Organism
Escherichia coli (strain K12)
Function
Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester (PubMed:25239919). In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible (PubMed:6347395 and PubMed:8940112).
Similarity
Belongs to the 2H phosphoesterase superfamily. ThpR family.
Sequence
MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHLTLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQLANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFARGRTRYTPLKRWALTQ