stsIR

Gene

stsIR

Protein

Type-2 restriction enzyme StsI

Organism

Streptococcus sanguinis

Length

602 amino acids

Function

Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 15 bases after G-1 and 14 bases before C-1.

Mass

68.392 kDa

Sequence

MTISINEYSDLNNLAFGLGQDVSQDLKELVKVASIFMPDSKIHKWLIDTRLEEVVTDLNLRYELKSVITNTPISVTWKQLTGTRTKREANSLVQAVFPGQCSRLAIVDWAAKNYVSVAVAFGLLKFHRADKTFTISEIGIQAVKLYDSEELAELDKFLYERLLEYPYAAWLIRLLGNQPSKQFSKFDLGEHFGFIDELGFETAPIEIFLNGLAQAEIDGDKTAAQKIKSNFESTSDKYMRWLAGVLVTAGLATSTTKKVTHTYKNRKFELTLGTVYQITAKGLTALKEVNGKSRYPRSRKRVMWEFLATKDKEAIAKKTSRSLMLKHLTEKKNPIQAEVIATLINTDYPTLEITPEEVIDDCIGLNRIGIEILIDGDKLTLNDKLFDFEIPVQKDVVLEKSDIEKFKNQLRTELTNIDHSYLKGIDIASKKKTSNVENTEFEAISTKIFTDELGFSGKHLGGSNKPDGLLWDDDCAIILDSKAYSEGFPLTASHTDAMGRYLRQFTERKEEIKPTWWDIAPEHLDNTYFAYVSGSFSGNYKEQLQKFRQDTNHLGGALEFVKLLLLANNYKTQKMSKKEVKKSILDYNISYEEYAPLLAEIE

Protein

Type-2 restriction enzyme StsI: stsIR