spnR

dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase

385 amino acids

Involved in the biosynthesis of forosamine ((4-dimethylamino)-2,3,4,6-tetradeoxy-alpha-D-threo-hexopyranose), a highly deoxygenated sugar component of several bioactive natural products such as the insecticidal spinosyns A and D (PubMed:11358695, PubMed:18345667). In the presence of pyridoxal 5'-phosphate (PLP) and alpha-ketoglutarate, catalyzes the C-4 transamination of dTDP-4-keto-2,3,6-trideoxy-alpha-D-glucose to yield dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-glucose (PubMed:18345667). It can also use pyruvate, but less efficiently than alpha-ketoglutarate (PubMed:18345667). Also able to catalyze the C-4 transamination of dTDP-4-keto-2,6-dideoxy-alpha-D-glucose to yield dTDP-4-amino-2,4,6-trideoxy-D-glucose (PubMed:18345667).

Belongs to the DegT/DnrJ/EryC1 family.

42.296 kDa

MINLHQPILGTEELDAIAEVFASNWIGLGPRTRTFEAEFAHHLGVDPEQVVFLNSGTAALFLTVQVLDLGPGDDVVLPSISFVAAANAIASSGARPVFCDVDPRTLNPTLDDVARAITPATKAVLLLHYGGSPGEVTAIADFCREKGLMLIEDSACAVASSVHGTACGTFGDLATWSFDAMKILVTGDGGMFYAADPELAHRARRLAYHGLEQMSGFDSAKSSNRWWDIRVEDIGQRLIGNDMTAALGSVQLRKLPEFINRRREIATQYDRLLSDVPGVLLPPTLPDGHVSSHYFYWVQLAPEIRDQVAQQMLERGIYTSYRYPPLHKVPIYRADCKLPSAEDACRRTLLLPLHPSLDDAEVRTVADEFQKAVEHHISQRSPLRK