Protein
Structure-specific endonuclease subunit SLX1 homolog
Organism
Caenorhabditis elegans
Function
Catalytic subunit of a heterodimeric structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA (Potential). Has a preference for replication forks over 5' flap structures or Holliday junctions and shows much lower activity toward 3' flap structures (PubMed:22927825). Required for proper crossover distribution through inhibition of crossover formation at the central region of chromosomes (PubMed:22927825, PubMed:23874210).
Similarity
Belongs to the SLX1 family.
Sequence
METFILSSDSDDDSGPPPSKRRTIEGIPRSFSGDKKIRFSFGASNMSQDTTQEIIPEDTDTVPLTIPSTPALSGKFDSKTPKSVRRRSVSMSCFTPIVETINQPPTNQACSSFLQKEYNFHDLSSDEEGGDGGAGCSKDEDKIIEDNLNLRALLSPEKKKRKEKIEEVQNEFYGVYCLISRSDRPCYKNRCYIGYTVDPNRRIMQHNGGRDKGGAKKTDSRGPWDMVCVVHGFPNHVAALHFEWAWQNPLVSKSLKEKQLRKERKETPFAFQLRIACELMNSSAFCRFALTFRWLITTEELPFPTSCVPPDHTKLRFGKVKKEMSLVPSKREDYLEMGECRICGKDIEKLWSLVRCISATCPSHFHSKCLSENGLKLKNEHVDHVYPLKANCPTCGQFYLWGDIIREQRRIIKISTKCAEEFQNMVVRKELPHREISPISSKK